CAJ | 학술논문

本文评述了用于作物品种鉴定的形态标记(morphological markers)、细胞标记(cytological markers)、生化标记(biochemical markers)、分子标记(molecular markers)的优缺点.重点评述了分子标记在作物品种鉴定中的应用.文中除对蛋白质电泳指纹图谱&&同工酶和贮藏蛋白(包括醇溶性蛋白、清蛋白、谷蛋白、球蛋白等)电泳产生的指纹图谱的应用外,较详细地介绍了近年来DNA指纹图谱技术;包括限制片段长度多态性(restriction fragment length polymorphism,简称RFLP)、随机扩增多态性DNA (random amplified polymorphic DNA,简称RAPD)、小卫星DNA(minisatellite DNA)、微卫星DNA(microsatellite DNA),简单重复序列间扩增(intersimple sequence repeats,简称ISSR),扩增片段长度多态性(amplified fragment length polymorphism,简称AFLP)以及CAPS (cleaved amplified polymorphic sequences)和SNPS (single nucleotide polymorphisms)对作物品种鉴定和新品种登记,品种纯度和真实性的检验以及品种间亲缘关系的探讨和在分类研究中的贡献等.
본문평술료용우작물품충감정적형태표기(morphological markers)、세포표기(cytological markers)、생화표기(biochemical markers)、분자표기(molecular markers)적우결점.중점평술료분자표기재작물품충감정중적응용.문중제대단백질전영지문도보&&동공매화저장단백(포괄순용성단백、청단백、곡단백、구단백등)전영산생적지문도보적응용외,교상세지개소료근년래DNA지문도보기술;포괄한제편단장도다태성(restriction fragment length polymorphism,간칭RFLP)、수궤확증다태성DNA (random amplified polymorphic DNA,간칭RAPD)、소위성DNA(minisatellite DNA)、미위성DNA(microsatellite DNA),간단중복서렬간확증(intersimple sequence repeats,간칭ISSR),확증편단장도다태성(amplified fragment length polymorphism,간칭AFLP)이급CAPS (cleaved amplified polymorphic sequences)화SNPS (single nucleotide polymorphisms)대작물품충감정화신품충등기,품충순도화진실성적검험이급품충간친연관계적탐토화재분류연구중적공헌등.
The article discussed the advantages and disadvantages of using morphological markers, cytological markers, biochemical markers and molecular markers in the identification of crop cultivars, focusing on the application of moleuclar marker fingerprint in identifying crop cultivars. In addition to the discussion of the application of protein eletrophoresis fingerprint, which is produced by the electrophoresis of isozyme and depot protein (prolamin, albumin, glutelin, globulin, etc.), recent DNA fingerprint techniques of RFLP, RAPD, minisatelite DNA, microsatellite DNA, ISSR, AFLP, CAPS and SNPS, and their contribution to the identification of crop cultivars, new cultivars registration, the inspection of cultivar purity, the relationship among cultivars and the studies in classification are thoroughly discussed as well.