CAJ | 학술논문

用RAPD技术对蓖麻蚕基因组DNA进行多态性研究，分析了5个蓖麻蚕品种间的遗传差异。结果表明，所采用的40个随机引物中，有27个引物扩增谱带清晰且重复性较好，扩增总片段数达243个，单个引物的扩增片段数在4～17之间，平均为9条，片段大小在0.33～3.0kb之间。不同蓖麻蚕品种间的遗传距离(D)在0.0683～0.1603之间，根据D值，由UPGMA聚类分析软件绘制了它们的聚类分子树。
용RAPD기술대비마잠기인조DNA진행다태성연구，분석료5개비마잠품충간적유전차이。결과표명，소채용적40개수궤인물중，유27개인물확증보대청석차중복성교호，확증총편단수체243개，단개인물적확증편단수재4～17지간，평균위9조，편단대소재0.33～3.0kb지간。불동비마잠품충간적유전거리(D)재0.0683～0.1603지간，근거D치，유UPGMA취류분석연건회제료타문적취류분자수。
Random amplified Polymorphic DNA (RAPD) was used to analyze the genetic diversity among eri sickworm. The genetic variance of five erisickworm was studied. The result showed that: 27 of 40 arbitrary primers could amplify clearly with repeatable bands.243 fragments were obtained.Each primer gave 4～17 bands and the average was 9.The length of the band was 0.33～3.0kb. The genetic distance (D) value between different breeds of Eri Silkworm was 0.0683～0.1603. The D value was used to construct a dendrogram by UPGMA.