CAJ | 학술논문

为了检测黑升麻（CR）的雌激素活性及其对人类乳腺癌MCF-7细胞雌激素受体（ERs）水平的影响，未成年雌性小鼠按体重以75、150和300mg/kg的CR灌胃14d，观察处于动情期的时间，第15天处死后取子宫和卵巢称重。以MTT实验筛选CR的最佳细胞作用剂量，观察对照组、CR组（4.75μg/L）和17β-雌二醇组（0.3nmol/L）的细胞生长曲线，并在流式细胞仪上用间接免疫荧光法测定各组ERs水平。结果发现子宫重量随CR剂量增加而增加，300mg/kg的CR可显著增加动情天数(P<0.05）。4.75μg/LCR对MCF-7细胞的促增殖作用最强，达64.7%；CR组和17β-雌二醇组的细胞群体倍增时间（TD）分别为32.1h和31.7h，明显低于对照组(TD=35.3h)；4.75μg/L的CR可显著增加细胞的ERs水平（P<0.01）。结果表明CR具有雌激素活性，其升高ERs水平的作用可能是该物质可治疗更年期综合征的机制之一。
위료검측흑승마（CR）적자격소활성급기대인류유선암MCF-7세포자격소수체（ERs）수평적영향，미성년자성소서안체중이75、150화300mg/kg적CR관위14d，관찰처우동정기적시간，제15천처사후취자궁화란소칭중。이MTT실험사선CR적최가세포작용제량，관찰대조조、CR조（4.75μg/L）화17β-자이순조（0.3nmol/L）적세포생장곡선，병재류식세포의상용간접면역형광법측정각조ERs수평。결과발현자궁중량수CR제량증가이증가，300mg/kg적CR가현저증가동정천수(P<0.05）。4.75μg/LCR대MCF-7세포적촉증식작용최강，체64.7%；CR조화17β-자이순조적세포군체배증시간（TD）분별위32.1h화31.7h，명현저우대조조(TD=35.3h)；4.75μg/L적CR가현저증가세포적ERs수평（P<0.01）。결과표명CR구유자격소활성，기승고ERs수평적작용가능시해물질가치료경년기종합정적궤제지일。
The estrogenicity of Black Cohosh (Cimicifuga racemosa, CR) wastested in vivo and in vitro and its effect on estrogen receptor (ER) level of human breast cancer MCF-7 cells were investigated. Based on the body weight of animals, 75, 150 and 300 mg/kg of CR were administered by tube feeding to immature female mice for 14 days. Estrus was observed and the uterine and ovary weights of mice were measured. The optimal dose of CR for the growth of MCF-7 cells was screened by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay. Subsequently, Growth curves of MCF-7 cells in blank control, 4.75μg/L of CR and 0.3nmol/L of 17β-estradiol groups were observed for 5 days. ER level in MCF-7 cells was analyzed by indirect immunofluorescence assay in flow cytometry. The results showed that uterine weight increased with the increasing dosage of CR and the days of estrus was significantly prolonged in the 300 mg/kg group (P<0.05).The concentration of CR at 4.75μg/L showed the strongest enhancement effects (64.7%). The doubling time (TD) of cell growth in CR group and 17β-estradiol group were 32.1 h and 31.7 h respectively, which were shorter than that of blank control (TD=35.3 h). Additionally, 4.75μg/L of CR significantly increased ER levels compared with the blank control (P<0.01). Taking all the results together, CR has an estrogen-like action. The enhancing effect of CR on ER level is one of the potential mechanisms involved with its therapeutic role in climacteric syndrome.