中国现代应用药学
中國現代應用藥學
중국현대응용약학
CONTENTS IN BRIEF
2001年
2期
124-126
,共3页
封士兰%陈立仁%赵富虎%赵文君%李建忠%柳对平
封士蘭%陳立仁%趙富虎%趙文君%李建忠%柳對平
봉사란%진립인%조부호%조문군%리건충%류대평
高效液相色谱法%五加%剌五加苷B%剌五加苷E
高效液相色譜法%五加%剌五加苷B%剌五加苷E
고효액상색보법%오가%랄오가감B%랄오가감E
目的：测定甘肃产五加中剌五加苷B、苷E的含量。方法：高效液相色谱法，ODSKromasal柱。水：乙晴(95：5)为流动相，检测波 长222nm,柱温度25℃。结果：本文可同时测定剌五加苷B、苷E的含量。 剌五加苷B、苷E分别在0.064～0.320μg/ml；0.074～0.370μg/ml范围内峰面积与浓度呈线 性关系，平均回收率分别为102.5%，RSD=4.2%,95.5%,RSD=4.6%。结论：剌五加苷B、苷E在红毛五加中含量最高；茎皮中含量最 高；剌五加苷E的含量高于苷B。
目的：測定甘肅產五加中剌五加苷B、苷E的含量。方法：高效液相色譜法，ODSKromasal柱。水：乙晴(95：5)為流動相，檢測波 長222nm,柱溫度25℃。結果：本文可同時測定剌五加苷B、苷E的含量。 剌五加苷B、苷E分彆在0.064～0.320μg/ml；0.074～0.370μg/ml範圍內峰麵積與濃度呈線 性關繫，平均迴收率分彆為102.5%，RSD=4.2%,95.5%,RSD=4.6%。結論：剌五加苷B、苷E在紅毛五加中含量最高；莖皮中含量最 高；剌五加苷E的含量高于苷B。
목적：측정감숙산오가중랄오가감B、감E적함량。방법：고효액상색보법，ODSKromasal주。수：을청(95：5)위류동상，검측파 장222nm,주온도25℃。결과：본문가동시측정랄오가감B、감E적함량。 랄오가감B、감E분별재0.064～0.320μg/ml；0.074～0.370μg/ml범위내봉면적여농도정선 성관계，평균회수솔분별위102.5%，RSD=4.2%,95.5%,RSD=4.6%。결론：랄오가감B、감E재홍모오가중함량최고；경피중함량최 고；랄오가감E적함량고우감B。
OBJECTIVE：To separate and detemine eleutherosi de B、E in Acathopanax.METHOD：A HPLC method was used with a ODS Kromasal column and the mobile phase consisting of MeOH and CN3CN(95:5).The d etection wavelength was at 222nm.RESUITS:The two compounds could be assayed simultaneously The Calibration curves were linear in the range of 0 .064～0.370μg/ml for eleutheroside E.The average recoveries were 102.5%(RSD=4.2%)、95.5%(RSD=4.6%) respectively.CONCLUSI ON:The content of eleutheroside B、 E is the most in A.giraldii.Their cont ents are also the most in bark,But the content of eleutheroside E is more than t haf of eleutheroside B in every sample.
