CAJ | 학술논문

对转Bt基因水稻与常规品种的杂交后代进行了GUS活性的组织化学分析和农艺性状考察。结果表明，报告基因gus与抗虫基因cry1Ab是连锁遗传和协同表达的，利用GUS组织化学染色法可快速筛选抗虫杂种植株；无论是籼粳交，还是粳粳交后代，抗虫和感虫的两类植株间在株高、穗长、单株分蘖数、播始历期和千粒重等主要农艺性状上无显著差异，这无疑为利用Bt水稻培育优良的抗虫品种提供了科学依据。
대전Bt기인수도여상규품충적잡교후대진행료GUS활성적조직화학분석화농예성상고찰。결과표명，보고기인gus여항충기인cry1Ab시련쇄유전화협동표체적，이용GUS조직화학염색법가쾌속사선항충잡충식주；무론시선갱교，환시갱갱교후대，항충화감충적량류식주간재주고、수장、단주분얼수、파시력기화천립중등주요농예성상상무현저차이，저무의위이용Bt수도배육우량적항충품충제공료과학의거。
GUS activity was analyzed by histochemical staining and agronomictraits were observed in hybrid progenies between transgenic Bt rice and conventional rice varieties. Results showed that reporter gene gus was co-inherited and co-expressed with the insect resistant gene cry1Ab and the GUS histochemical assay can be used to rapidly screen insect resistant plants. And no significant differences in main agronomic traits, such as plant height, panicle length, the number of tillers per plant, days to heading and 1000-grain weight, were found between the two classes of the insect resistant plant and the susceptible plant in the progenies of either indica/japonica crosses or japonica/japonica crosses, providing scientific basis for utilization of transgenic Bt rice in cross breeding for elite varieties with insect resistance.