CAJ | 학술논문

目的建立一种柱上样品堆积法测定微量氨基酸的方法。方法分别用运行缓冲液或用水和乙腈重组经萃取吹干的样品，压力法进样5、25和50 s，高效毛细管电泳法定量分析，观察用不同溶剂重组对柱上样品堆积效果的影响。结果该法提高微量氨基酸检测灵敏度100倍并保持柱效不变。结论用水和乙腈(1∶1)作溶剂重组残留样品的方法获得最佳的柱上堆积效果。
목적건립일충주상양품퇴적법측정미량안기산적방법。방법분별용운행완충액혹용수화을정중조경췌취취간적양품，압역법진양5、25화50 s，고효모세관전영법정량분석，관찰용불동용제중조대주상양품퇴적효과적영향。결과해법제고미량안기산검측령민도100배병보지주효불변。결론용수화을정(1∶1)작용제중조잔류양품적방법획득최가적주상퇴적효과。
Purpose　The aim is to develop a method for the analysis of micro-scale amino acids in biological samples.Methods　After having been extracted and dried,the residues were dissolved in a running buffer or a distilled water and acetonitrile.The samples were loaded into column for 5,25 or 50s at a hydrodynamic injection and were separated by capillary electrophoresis.The effect of different dissolvent on the sample stacking was observed.Results　A 100-fold improvement in the amount of material that can be injected into a capillary column without loss of resolution was shown.Conclusion　The marked effect of sample stacking was achieved by the using distilled water and acetonitrile(1∶1,v/v) as dissolvent.