CAJ | 학술논문

在酸浸出法测定镉含量的基础上，用超声处理血液样品，建立了一种简便的石墨炉原子吸收分光光度法来测定血液样品中的微量砷。通过不同基体改进剂的比较，确定了镍作为测定砷的基体改进剂，1.0mL血液样品用0.1mL 8mol/L HCl浸泡样品，超声处理30分钟后测定，其检出限为3.03ng/mL；线性范围为5.0～50.0ng/mL；精密度为2.5%；回收率在88.0%～98.1%之间。研究表明该法测定血液中的微量砷，具有简单、灵敏、精密、准确等特点。用本法测定了慢性砷中毒小鼠红细胞中的砷含量，结果表明中毒组小鼠红细胞中砷含量显著高于对照组。
재산침출법측정력함량적기출상，용초성처리혈액양품，건립료일충간편적석묵로원자흡수분광광도법래측정혈액양품중적미량신。통과불동기체개진제적비교，학정료얼작위측정신적기체개진제，1.0mL혈액양품용0.1mL 8mol/L HCl침포양품，초성처리30분종후측정，기검출한위3.03ng/mL；선성범위위5.0～50.0ng/mL；정밀도위2.5%；회수솔재88.0%～98.1%지간。연구표명해법측정혈액중적미량신，구유간단、령민、정밀、준학등특점。용본법측정료만성신중독소서홍세포중적신함량，결과표명중독조소서홍세포중신함량현저고우대조조。
A simple method of determining arsenic in blood by electrothermal atomic absorption spectrophotometer was developed based on the modification of the method of extraction with acid. A Ni(NO3)2 matrix modifier was used to thermally stabilize arsenic. After comparing to the wet-digestion method, the result suggests that this method is relatively simple, accurate, precise and sensitive. The detection limit is 3.03 ng/mL, the precision is 2.5% and the recovery in the range of 88.0%～98.1%. The concentration of arsenic in blood was determined by this method. The results show that the concentration of arsenic in blood of the arsenism mice is higher than that in the control mice.