华中农业大学学报
華中農業大學學報
화중농업대학학보
JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
2001年
2期
159-163
,共5页
曹少先%杨利国%赵文魁%张兆柏%姜勋平%茆达干
曹少先%楊利國%趙文魁%張兆柏%薑勛平%茆達榦
조소선%양리국%조문괴%장조백%강훈평%묘체간
波尔山羊%随机扩增多态DNA(RAPD)%体重%体尺
波爾山羊%隨機擴增多態DNA(RAPD)%體重%體呎
파이산양%수궤확증다태DNA(RAPD)%체중%체척
应用19个波尔山羊个体间多态性较强、重复性好的随机引物对20只5~6月龄波尔山羊公羊组织样DNA进行扩增,共得到156个标记,其中可变标记47个。t检验表明,OPE15.1阳性组腰角宽显著高于阴性组,OPH15.8、OPE15.4阳性组臀端宽显著高于阴性组;而OPH 13.6、OPH13.8等标记阴性组多个体尺、体重性状显著高于阳性组。这说明这些标记与特定性状可能存在相关,其遗传基础可能是该标记与控制性状的QrL或主基因连锁。
應用19箇波爾山羊箇體間多態性較彊、重複性好的隨機引物對20隻5~6月齡波爾山羊公羊組織樣DNA進行擴增,共得到156箇標記,其中可變標記47箇。t檢驗錶明,OPE15.1暘性組腰角寬顯著高于陰性組,OPH15.8、OPE15.4暘性組臀耑寬顯著高于陰性組;而OPH 13.6、OPH13.8等標記陰性組多箇體呎、體重性狀顯著高于暘性組。這說明這些標記與特定性狀可能存在相關,其遺傳基礎可能是該標記與控製性狀的QrL或主基因連鎖。
응용19개파이산양개체간다태성교강、중복성호적수궤인물대20지5~6월령파이산양공양조직양DNA진행확증,공득도156개표기,기중가변표기47개。t검험표명,OPE15.1양성조요각관현저고우음성조,OPH15.8、OPE15.4양성조둔단관현저고우음성조;이OPH 13.6、OPH13.8등표기음성조다개체척、체중성상현저고우양성조。저설명저사표기여특정성상가능존재상관,기유전기출가능시해표기여공제성상적QrL혹주기인련쇄。
DNA samples were obtained from tissue of 20 stud Boer goats aged 5~6 months and amplified by 19 selected primers and 156 markers including 47 variable markers were observed in the present study. The results suggested that hip width mean of marker OPE 15.1 positive goat group was significantly higher than that of the negative group, and pin bone width mean of markers OPE 15.4 and OPH 15.8 positive goat group was significantly higher than that of the negative group, while phenotype mean of several traits of markers OPH 13.6 and OPH 13.8 etc negative goat group was significantly higher than that of the positive group . This suggested that these markers and some special traits might be correlative, and the genetic mechanism might be that these markers are linked with QTL or main gene controlled some special traits.