CAJ | 학술논문

报道了一种适合粉纹夜蛾(5B1)细胞生长的无血清培养基(简称为SFM).该培养基以Tc-100基础培养基为基础，补加一定量的水解乳蛋白和酵母抽提物，并加入适量的微量元素、维生素、脂类复合物等，5B1细胞已在该SFM中传代18代，研究结果表明：该培养基能支持5B1细胞的生长和芹菜夜蛾核型多角体病毒(SfaMNPV)在其中复制.
보도료일충괄합분문야아(5B1)세포생장적무혈청배양기(간칭위SFM).해배양기이Tc-100기출배양기위기출，보가일정량적수해유단백화효모추제물，병가입괄량적미량원소、유생소、지류복합물등，5B1세포이재해SFM중전대18대，연구결과표명：해배양기능지지5B1세포적생장화근채야아핵형다각체병독(SfaMNPV)재기중복제.
This paper described a new kind of serum-free medium. The medium was based on TC-100 medium and supplemented with 10% lactalbumin hydrolysate and yeast extract, proper trace elements, vitamins, lipid concentration and etc. 5B1 cell, had been subcultured 18 times in SFM.The results showed the medium was able to support the growth of 5B1 cell and the replication of SfaMNPV in 5B1 cells.