CAJ | 학술논문

目的探讨宫颈癌组织MDR1和GST-π基因的表达和临床意义。方法应用RT- PCR方法检测了20例宫颈癌组织耐药基因MDR1和GST-π。结果宫颈癌组织MDR1、GST-π阳性表达率分别为65％(13／20)、75％(15／20)，MDR1和GST-π共表达率为50％(10／20)，二者均表达阴性2例(10％)。两种耐药基因表达与肿瘤分化、临床分期均无关。结论宫颈癌组织中亦存在较高的MDR1和GST-π基因表达，而二者在其先天性耐药机制中均占有重要的作用，这对今后治疗具有一定的参考价值。
목적탐토궁경암조직MDR1화GST-π기인적표체화림상의의。방법응용RT- PCR방법검측료20례궁경암조직내약기인MDR1화GST-π。결과궁경암조직MDR1、GST-π양성표체솔분별위65％(13／20)、75％(15／20)，MDR1화GST-π공표체솔위50％(10／20)，이자균표체음성2례(10％)。량충내약기인표체여종류분화、림상분기균무관。결론궁경암조직중역존재교고적MDR1화GST-π기인표체，이이자재기선천성내약궤제중균점유중요적작용，저대금후치료구유일정적삼고개치。
Objective To detect the levels of MDR1 and GST-π gene expression in cervical cancer tissues. Methods The expression of MDR1 and GST-π gene in 20 cervical cancer tissues were studied by reverse transcriptionpolymerase chain reaction(PT-PCR). Results Thirteen of the 20 cervical cancer tissues (65%) were positive expression of MDR1 gene, while in 15 of the 20 cervical cancer tissues (75%) GST-π gene expressed. In 10 of the 20 cervical cancer specimens (50%) MDR1 and GST-π gene co-expressed, while in 2 of the 20 cervical cancer specimens (10%) were negative co-expression of MDR1 and GST-π gene. No correlations were noted between MDR1/GST-π gene expression with histological differentiation and clinical stage. Conclusion The results suggest that MDR1 and GST-π gene might be taken as tumor markers of cervical cancer and that coexpression of MDR1 and GST-π gene is of significance in the mechanism of drug resistance of cervical cancer.