CAJ | 학술논문

目的：通过对饮食从业人员外周血T淋巴细胞亚群、B淋巴细胞数量分析来了解烹调烟雾对机体免疫功能的影响。方法：采用直接荧光法，通过流式细胞仪分析人外周血T淋巴细胞亚群、B淋巴细胞数量。结果：经成组t检验，作为烹调烟雾暴露组的红案工外周血T淋巴细胞百分率明显低于对照组白案工，工龄在7年以上被调查者中红案工CD4+CD8-细胞百分率、CD4+/CD8+细胞之值明显低于白案工，而B淋巴细胞百分率两组之间差异无统计学意义；多因素逐步回归分析表明，暴露与否是影响T淋巴细胞总数的主要因素且与工龄有关。结论：长期暴露于烹调烟雾对机体的细胞免疫功能有一定抑制作用。
목적：통과대음식종업인원외주혈T림파세포아군、B림파세포수량분석래료해팽조연무대궤체면역공능적영향。방법：채용직접형광법，통과류식세포의분석인외주혈T림파세포아군、B림파세포수량。결과：경성조t검험，작위팽조연무폭로조적홍안공외주혈T림파세포백분솔명현저우대조조백안공，공령재7년이상피조사자중홍안공CD4+CD8-세포백분솔、CD4+/CD8+세포지치명현저우백안공，이B림파세포백분솔량조지간차이무통계학의의；다인소축보회귀분석표명，폭로여부시영향T림파세포총수적주요인소차여공령유관。결론：장기폭로우팽조연무대궤체적세포면역공능유일정억제작용。
Objective　To inquire the effects of cooking oil fumes on the immunologic function by determining T and B lymphocyte subtypes in peripheral blood of cooking workers.Method　T and B lymphocyte were determined by immunofluorescence assay through fluorescence activated cell sorter(FACS).Results　It was showed that the proportion of T cells in the exposed group were obviously lower than those in the controls.Meanwhile,the same results were exhibited on the percentage of CD4+CD8- T cells,the CD4+/CD8+ ratio between the exposed group working longer than 7 years and controls.The proportion of B cells,however,showed no difference between the two groups.Stepwise regression analysis indicated that exposure or not was the main influence factor on the T cells,CD4+CD8- T cells,CD4+/CD8+ ratio,meanwhile,it showed that the proportion of T cells was related to years of working.Conclusion　The cell-mediated immunologic function is inhibited by touching with a lot of cooking oil fumes with long time.