CAJ | 학술논문

①目的探讨银屑病的发病与细胞凋亡以及细胞凋亡与皮损组织锌含量的关系。②方法采用末端脱氧核苷酰转移酶（TdT）介导的d-UTP生物素缺口末端标记（TUNEL）技术、流式细胞技术检测银屑病病人皮损组织中凋亡细胞，用原子吸收分光法检测银屑病皮损组织锌的含量。③结果银屑病病人皮损组织中细胞发生凋亡的比例高于正常对照组(t=4.02,4.53,P<0.01), 银屑病病人皮损组织锌含量明显低于对照组 (t=4.31,P<0.01),皮损组织中细胞凋亡数与锌含量呈负相关(r=-0.423,P<0.05)。④结论锌可能对银屑病皮损组织中细胞凋亡有明显的影响。
①목적탐토은설병적발병여세포조망이급세포조망여피손조직자함량적관계。②방법채용말단탈양핵감선전이매（TdT）개도적d-UTP생물소결구말단표기（TUNEL）기술、류식세포기술검측은설병병인피손조직중조망세포，용원자흡수분광법검측은설병피손조직자적함량。③결과은설병병인피손조직중세포발생조망적비례고우정상대조조(t=4.02,4.53,P<0.01), 은설병병인피손조직자함량명현저우대조조 (t=4.31,P<0.01),피손조직중세포조망수여자함량정부상관(r=-0.423,P<0.05)。④결론자가능대은설병피손조직중세포조망유명현적영향。
Objective To explore the relationship between zinc and apoptosis of keratinocytes in psoriatic lesion. Methods Apoptosis of keratinocytes in psoriatic lesion and normal skin were measured by both terminal deoxynucleotidyl transferase-mediation dUTP-biotin nick end labelling (TUNEL) and flow cytometer. The zinc contents in psoriatic lesion and normal skin were measured by atomic absorption spectrophotometry(AAS). Results The zinc contents in psoriatic lesion were lower(t=4.31，P<0.01) and the number of apoptotic keratinocytes was higher than that of the normal skin(t= 4.02,4.53,P<0.01).There was a negative correlation between the contents of zinc and the percentage of apoptotic keratinocytes in psoriatic lesion(r=-0.423,P<0.05). Conclusion Zinc may play an important role in keratinocytes apoptosis in patients with psoriasis.