CAJ | 학술논문

目的与方法：建立高效液相色谱内标法测定人血清中头孢克罗浓度。以甲醇为蛋白沉淀剂，头孢拉定为内标，流动相为甲醇：醋酸缓冲液：二乙胺(15:85:0.5 V/V/V)，检测波长265 nm，流速1.0 m1/min，记录纸速025 cm/min。结果：浓度在0.25～35μg/ml范围内呈良好线性关系，标准曲线的回归方程为Y=0.065 6+0.041 3X，相关系数r=0.999 3，萃取回收率为83.6％，最低检测浓度0.125μg/ml。结论：本法简单、快速，日内和日间回收率和精密度试验的相对标准差(RSD)均<6％，重复性好
목적여방법：건립고효액상색보내표법측정인혈청중두포극라농도。이갑순위단백침정제，두포랍정위내표，류동상위갑순：작산완충액：이을알(15:85:0.5 V/V/V)，검측파장265 nm，류속1.0 m1/min，기록지속025 cm/min。결과：농도재0.25～35μg/ml범위내정량호선성관계，표준곡선적회귀방정위Y=0.065 6+0.041 3X，상관계수r=0.999 3，췌취회수솔위83.6％，최저검측농도0.125μg/ml。결론：본법간단、쾌속，일내화일간회 수솔화정밀도시험적상대표준차(RSD)균<6％，중복성호
Aim and Method:A internal standard high-performance liquid chromatography for the determination of cefalor in human serum has been developed. Using cephradine as internal standard, proteins were precipitated with methanol. The supernatant was performed in an YWGc 18 column with a mobile phase of methanol:acetic-sodium acetate buffer:biethylamine(15: 85: 0. 5 V/V/V)and detected wave at 265 nm,flow rate was 1.0 ml/min. Results: The linear concentratiion range of this method was 0. 25～35 μg/ml and the detectable lowest concentration was 0. 125 μg/ml; the regressive equation of standard curve was Y=0. 065 6＋0. 041 3 X; correlative coefficient r=0. 999 3 and extractive efficiency was 83. 69%. Conclusion.. This is a rapid、sensitive and reproducible method for determination of Cefaclor in human serum. Relative standard difference(RSD)of both recovery and precision were under 6% for within-day and day-to-day.