CAJ | 학술논문

目的：探讨痰聚合酶链反应(PCR)TB-DNA、血清中结核分支杆菌胞壁糖脂免疫球蛋白C(LAM IgG)、卡介苗免疫球蛋白G(PPD IgG)及结核特异循环免疫复合物(SCIC)检测与结核菌素(PPD 0.1u)皮试，联合检测共同阳性对菌阴肺结核的诊断价值。方法：病材取自初治菌阴肺结核31例，健康对照组53例，非结核肺疾病30例，初治菌阴肺结核54例。采用同步检测方法。血清免疫学检测用酶联免疫吸附试验(ELISA)观察各组共同阳性率的变化，对结果进行分析评价。结果：健康对照组2、3、4、5联阳性率均为0，特异性为100％；初治菌阳组2、3、4、5联同时阳性率分别为67.7％，58.1％，32.3％，0％；初治菌阴组2、3、4、5联共同阳性率分别为45.9％，18.9％，2.7％，0％：非结核肺疾病组2、3、4、5联合同时阳性率分别为21.7％，13.0％，0％，0％。结论：五种方法联合对健康组2、3、4、5联同时阳性检测特异性可达100％，初治菌阴组阳性检出率2联可达45.9％，比任何单项方法检出率要高，由于原五种方法联合检测特异性有所下降，因此该方法更适用于初治菌阴肺结核的诊断，值得推广。
목적：탐토담취합매련반응(PCR)TB-DNA、혈청중결핵분지간균포벽당지면역구단백C(LAM IgG)、잡개묘면역구단백G(PPD IgG)급결핵특이순배면역복합물(SCIC)검측여결핵균소(PPD 0.1u)피시，연합검측공동양성대균음폐결핵적진단개치。방법：병재취자초치균음폐결핵31례，건강대조조53례，비결핵폐질병30례，초치균음폐결핵54례。채용동보검측방법。혈청면역학검측용매련면역흡부시험(ELISA)관찰각조공동양성솔적변화，대결과진행분석평개。결과：건강대조조2、3、4、5련양성솔균위0，특이성위100％；초치균양조2、3、4、5련동시양성솔분별위67.7％，58.1％，32.3％，0％；초치균음조2、3、4、5련공동양성솔분별위45.9％，18.9％，2.7％，0％：비결핵폐질병조2、3、4、5연합동시양성솔분별위21.7％，13.0％，0％，0％。결론：오충방법연합대건강조2、3、4、5련동시양성검측특이성가체100％，초치균음조양성검출솔2련가체45.9％，비임하단항방법검출솔요고，유우원오충방법연합검측특이성유소하강，인차해방법경괄용우초치균음폐결핵적진단，치득추엄。
Objective: To evaluate the diagnostic value of common positive of 5 kinds of methods(sputum PCR TB-DNA, serumLAM IgG, PPD IgG, SCIC and PPD 0.1 U skin test) used jointly for smear and culture negative pulmonary tuberculosis. Meth-ods: smear and culture positive cases 31,healthy control cases 53,non-tuberculosis Iung disease cases 30,smear and culturenegative cases 54. Methods were used for every case synchronousely The sputum were detection with PCR-TB-DNA, serum withELISA and PPD with skin test. Results: The positive rate(2,3,4,5 methods common positive)is 0,specificity is 100% in thehealth control group; the common positive rates among 2,3,4,5 methods respectively are 67.2% ,58.1% ,32.3% ,0% insmear and culture positive group; 45.9%, 18.9%, 2.7%, 0% in smear and culture negative group and 21.7%, 13.0%,0% ,0% in the non-tuberculosis pulmonary diseases group. Conclusion: The specificity of common positive rate of combina-tion detection of 5 kinds of methods is 100% in healthy control group; in smear and culture negative group the positive rate ofany 2 combination detections among 5 methods is 45.9% ,higher than any single detection of 5 methods. So those methods areavailable to smear and culture negative tuberculous diagnosis.