CAJ | 학술논문

目的确定氟化物及钙对卵巢切除大鼠血清脂质过氧化物的影响.方法 64只雌性Wistar大鼠,随机分为对照组,卵巢切除组,卵巢切除后单纯喂给氟组及卵巢切除后喂给氟加钙组.单纯给氟组卵巢切除后隔日喂给含氟化钠的水,氟离子的浓度分别是2、4、6mg/L,氟加钙组则在喂给氟同时又加入等剂量的900mg/L的葡萄糖酸钙.喂养6个月后,分组处死动物,用TBA法测定血清中的脂质过氧化物含量.结果卵巢切除组(OVX组)大鼠血清中LPO含量明显高于假卵巢切除组(Sham组)(P＜0.05).卵巢切除同时喂给不同剂量的氟及氟加钙组,即OVXF2、OVXF4、OVXF6 、OVXF2Ca、OVXF4Ca、OVXF6Ca组大鼠血清LPO明显低于单纯卵巢切除组(OVX组)(P＜0.01).卵巢切除同时投给不同剂量的氟,即 OVXF2、 OVXF4、 OVXF6组与卵巢切除同时投给不同剂量的氟加钙,即OVXF2Ca、OVXF4Ca、OVXF6Ca组比较大鼠血清LPO改变不明显(P＞0.05).结论卵巢切除骨质疏松大鼠血中LPO明显增高,加入不同剂量的氟及钙后改善了去势大鼠的脂质过氧化程度.提示氟化物及氟化物加钙在治疗骨质疏松方面可能提高了机体的抗氧化能力.
목적학정불화물급개대란소절제대서혈청지질과양화물적영향.방법 64지자성Wistar대서,수궤분위대조조,란소절제조,란소절제후단순위급불조급란소절제후위급불가개조.단순급불조란소절제후격일위급함불화납적수,불리자적농도분별시2、4、6mg/L,불가개조칙재위급불동시우가입등제량적900mg/L적포도당산개.위양6개월후,분조처사동물,용TBA법측정혈청중적지질과양화물함량.결과란소절제조(OVX조)대서혈청중LPO함량명현고우가란소절제조(Sham조)(P＜0.05).란소절제동시위급불동제량적불급불가개조,즉OVXF2、OVXF4、OVXF6 、OVXF2Ca、OVXF4Ca、OVXF6Ca조대서혈청LPO명현저우단순란소절제조(OVX조)(P＜0.01).란소절제동시투급불동제량적불,즉 OVXF2、 OVXF4、 OVXF6조여란소절제동시투급불동제량적불가개,즉OVXF2Ca、OVXF4Ca、OVXF6Ca조비교대서혈청LPO개변불명현(P＞0.05).결론란소절제골질소송대서혈중LPO명현증고,가입불동제량적불급개후개선료거세대서적지질과양화정도.제시불화물급불화물가개재치료골질소송방면가능제고료궤체적항양화능력.
Objective To evaluate the effect of fluoride and fluoride plus calcium on serum LPO in ovariectomized rats. Methods Sixty-four female Wistar rats of three months were divided randomly into sham-operated group (sham), ovary resection group (OVX), after ovary resection given fluoride group (OVXF), and after ovary resection given fluoride plus calcium group (OVXFCa).Fluoride-added water was given in OVXF group after ovary resection once every other day, the fluorine content in water was 2mg、4mg、6mg per liter. OVXFCa group received the same fluoride as OVXF group, simultaneously with calcium gluconate, in each group, calcium ion content in water was 900mg per liter. After six months feeding, all animals were killed, and serum LPO was tested by TBA assay.Results There was a significant increase in serum LPO (nmol/L) in OVX group as compared with the sham group (P＜0.05 ). The content of serum LPO in OVXF and OVXFCa group was significantly lower than that in OVX group (P＜0.001),but there was no distinct difference between OVXF group and OVXFCa group in serum LPO(P＞0.05).Conclusion The content of rat serum LPO in ovary resection group increased remarkably and the lipid peroxidation degree of ovariectomized rats could be improved after given different dose fluoride and different dose fluoride plus calcium. It is suggested that fluoride and fluoride plus calcium may improve the antioxide capability of human body in curing osteoporosis.