CAJ | 학술논문

目的：观察卡介苗接种对高盐负荷及肾动脉狭窄所致高血压的影响，为寻找防治人类高血压的新方法提供实验依据。方法：以高盐食物喂饲及肾动脉狭窄术分别制作高盐性高血压（SH）及肾性高血压（RH）模型。高盐喂饲或肾动脉狭窄术后4周，选择高血压大鼠及正常对照大鼠进行实验。所有大鼠腹部皮下接种卡介苗一次，接种前及接种后每周测定血压变化情况，同时每时段收集24 h尿样并以Greiss反应法进行一氧化氮终产物NO－2及NO－3（UNOx）的测定。3周后大鼠分别取主动脉组织并用Western blot法检测iNOS蛋白水平。结果：卡介苗接种对正常大鼠血压无明显影响。但接种1周时，能使高血压大鼠的血压明显下降。其中尤以卡介苗接种＋L－精氨酸作用明显。NO测定表明，卡介苗接种后体内NO生成明显增加。Western blot结果证明卡介苗接种后，高血压大鼠主动脉血管组织中iNOS蛋白明显高于正常大鼠。结论：卡介苗接种能通过激活iNOS/NO途径降低某些类型高血压大鼠的血压。
목적：관찰잡개묘접충대고염부하급신동맥협착소치고혈압적영향，위심조방치인류고혈압적신방법제공실험의거。방법：이고염식물위사급신동맥협착술분별제작고염성고혈압（SH）급신성고혈압（RH）모형。고염위사혹신동맥협착술후4주，선택고혈압대서급정상대조대서진행실험。소유대서복부피하접충잡개묘일차，접충전급접충후매주측정혈압변화정황，동시매시단수집24 h뇨양병이Greiss반응법진행일양화담종산물NO－2급NO－3（UNOx）적측정。3주후대서분별취주동맥조직병용Western blot법검측iNOS단백수평。결과：잡개묘접충대정상대서혈압무명현영향。단접충1주시，능사고혈압대서적혈압명현하강。기중우이잡개묘접충＋L－정안산작용명현。NO측정표명，잡개묘접충후체내NO생성명현증가。Western blot결과증명잡개묘접충후，고혈압대서주동맥혈관조직중iNOS단백명현고우정상대서。결론：잡개묘접충능통과격활iNOS/NO도경강저모사류형고혈압대서적혈압。
AIM: The present study was designed to examine the effect of Bacillus Calmette-Guerin (BCG) vaccination on blood pressure, nitric oxide (NO) production and iNOS expression in hypertensive rats. METHODS: Renal hypertension (RH) were made by renal artery stenosis in SD rats and the sodium induced hypertensive (SH) rats were made by feeding the rats with high sodium chloride diet (5 g NaCl/100 g food). After four weeks, the hypertensive animals were subjected to the experiment. All the rats were vaccinated with BCG (0.1 mL, i.d) and blood pressure were examined every week. Greiss reaction was used to measure the urinary NO excretion and Western blot was applied to probe the iNOS protein expression in aortic tissue. RESULTS: It was shown that one week after BCG vaccination, the blood pressure decreased significantly in hypertensive rats induced by NaCl-overloading and renal artery stenosis, but not in normotensive control rats. Furthermore, the hypotensive effect of BCG vaccination was enhanced by co-administration of L-arginine. A significant increase in NO production was observed in hypertensive rats. Also, Western blot showed BCG vaccination led to an obvious increase in iNOS expression in the aortic tissue of hypertensive, but not of normal control rats. CONCLUSION: BCG vaccination could lower the blood pressure of hypertensive rats through activation of iNOS/NO pathway.