CAJ | 학술논문

朊病毒(Prion)蛋白是人和动物慢性中枢神经系统退化病的传染源，该蛋白的113-120序列被认为在其致病和传染机理中起着重要作用。以反相高效液相色谱为分析手段，研究了Prion蛋白113-120序列多肽的色谱保留行为。通过比较不同温度、不同流动相条件下该多肽色谱保留行为的变化，发现在以乙腈溶液为流动相时，lnKw随温度的变化关系和Van′t Hoff 曲线均比较简单，说明该多肽在乙腈溶液中所采取的构象均较稳定，不易受温度的影响。以甲醇溶液为流动相时，具有游离末端的多肽的lnKw随温度变化关系和Van′t Hoff曲线比末端羧基和氨基分别被酰胺封闭的多肽要复杂，说明具有游离末端的多肽在甲醇溶液中所采取的构象相对较不稳定，易受环境的影响。这些结果进一步证明，113-120序列在Prion蛋白构象变化中可能起着重要作用。
원병독(Prion)단백시인화동물만성중추신경계통퇴화병적전염원，해단백적113-120서렬피인위재기치병화전염궤리중기착중요작용。이반상고효액상색보위분석수단，연구료Prion단백113-120서렬다태적색보보류행위。통과비교불동온도、불동류동상조건하해다태색보보류행위적변화，발현재이을정용액위류동상시，lnKw수온도적변화관계화Van′t Hoff 곡선균비교간단，설명해다태재을정용액중소채취적구상균교은정，불역수온도적영향。이갑순용액위류동상시，구유유리말단적다태적lnKw수온도변화관계화Van′t Hoff곡선비말단최기화안기분별피선알봉폐적다태요복잡，설명구유유리말단적다태재갑순용액중소채취적구상상대교불은정，역수배경적영향。저사결과진일보증명，113-120서렬재Prion단백구상변화중가능기착중요작용。
Prions are the infectious agents of the transmissible spongiform encephalopathies. The sequence 113-120 of Prion proteins is thought to be the most highly amyloidogenic peptide. The chromatographic retention behaviors of Prion 113-120 peptide on C18-column were studied under reversed-phase high-performance liquid chromatographic conditions with isocratic elution. When aquo-acetonitrile mobile phases were used, the temperature-depending relationships of lnkw and Van′t Hoff plots of both peptides with free termini and with capped termini were simple. The results demonstrated that the conformations adopted by both peptides in aquo-acetonitrile mobile phases were relatively stable, and could not be perturbed by temperature. When aquo-methanol mobile phases were used, the temperature-depending relationships of lnkw and Van′t Hoff plots of peptide with free termini were more complicated than that of peptide with capped termini. These results demonstrated that the conformations adopted by peptide with free termini in aquo-methanol mobile phases were relatively unstable and could be perturbed by circumstance. All these results further demonstrate that the sequence 113-120 could play an important role in the conformational change of Prion proteins.