CAJ | 학술논문

对棉铃虫幼虫神经细胞进行了离体培养，并建立了其体外培养的实用方法.神经细胞在增补棉铃虫幼虫血淋巴的TC-100培养基中可贴壁生长，存活5～7d，培养基中不加血淋巴，而增补谷氨酰胺时，细胞也可贴壁生长，但接种24h后出现老化现象.增补适量葡萄糖，细胞可存活22～28d.细胞存活的最适pH值为6.4.90%的细胞具多条突起，与在体内生长有明显区别.棉铃虫抗性种群、对照种群、同种群不同龄期幼虫以及同一个体胸、腹神经节的神经细胞体外培养时形态无明显差异，但抗性品系幼虫运动神经元内线粒体明显增多，且神经围鞘与神经节膜厚度之比明显增加.
대면령충유충신경세포진행료리체배양，병건립료기체외배양적실용방법.신경세포재증보면령충유충혈림파적TC-100배양기중가첩벽생장，존활5～7d，배양기중불가혈림파，이증보곡안선알시，세포야가첩벽생장，단접충24h후출현노화현상.증보괄량포도당，세포가존활22～28d.세포존활적최괄pH치위6.4.90%적세포구다조돌기，여재체내생장유명현구별.면령충항성충군、대조충군、동충군불동령기유충이급동일개체흉、복신경절적신경세포체외배양시형태무명현차이，단항성품계유충운동신경원내선립체명현증다，차신경위초여신경절막후도지비명현증가.
The central neurons of Helicoverpa armigera (Hübner) were isolated from the ganglia of thorax and abdomen in vitro. Comparative studies on the ultrastructre of ventral nerve cord from the larvae were carried out. The results indicate that cell survived and grew fast in TC-100 insect cell culture medium supplemented with glutamine, glutathione and glucose. Higher concentration of glucose in the medium is necessary to the long term culture. The primary culture was improved by the addition of nature hemolymph collected from 5th-stage bollworm larvae. The cell survival and adherence to the substrate could be improved by controlling the total tissue volume per unit medium volume. Though the hemolymph pH of larvae is 6.8, the neurons could survive and grow in the condition of pH 6.2～7.0 (optimum pH is 6.4). Most of the cultured cells became multipolar but a few were monopolar like their in vivo counterparts. The ultrastructure studies show that the number of mitochondria in the motor neurons from resistant strain are higher than those of the susceptible strain, and the thickness of neural lamella and perineurium of resistant strain is thicker than those of the susceptible insects.