CAJ | 학술논문

用水迷宫和一次性被动回避反应两种行为学训练方法,结合脑室内注射一氧化氮合酶(NOS)抑制剂NAME(Nω-nitro-L-arginine)和原位杂交技术,观察了阻断NOS前后大鼠海马和大脑皮层前额叶等区域由行为学训练诱发的生长抑素(somatostatin,SOM)mRNA阳性细胞数量的改变.结果显示:(1)同未经训练的对照组相比,两种行为学训练都引起海马和大脑皮层中SOM mRNA阳性细胞的显著增加;(2)在脑室中注射了NAME的实验组动物,两种行为学训练都不能再诱发上述阳性细胞的增加,同时NAME也阻止了训练组出现的学习和记忆的形成.以上结果提示,一氧化氮参与了作为脑内学习和记忆神经化学基础之一的生长抑素表达增加的调控.
용수미궁화일차성피동회피반응량충행위학훈련방법,결합뇌실내주사일양화담합매(NOS)억제제NAME(Nω-nitro-L-arginine)화원위잡교기술,관찰료조단NOS전후대서해마화대뇌피층전액협등구역유행위학훈련유발적생장억소(somatostatin,SOM)mRNA양성세포수량적개변.결과현시:(1)동미경훈련적대조조상비,량충행위학훈련도인기해마화대뇌피층중SOM mRNA양성세포적현저증가;(2)재뇌실중주사료NAME적실험조동물,량충행위학훈련도불능재유발상술양성세포적증가,동시NAME야조지료훈련조출현적학습화기억적형성.이상결과제시,일양화담삼여료작위뇌내학습화기억신경화학기출지일적생장억소표체증가적조공.
The effects of nitric oxide(NO) on expression of somatostatin (SOM) messenger RNA (mRNA) following trainings of the Morris water maze task or one-trial passive avoidance test were investigated in rats by using in situ hybridization technique. Intracerebroventricular (i.c.v.) injection of saline vehicle or of 5 μmol Nω-nitro-L-arginine (NAME), an inhibitor of NO synthase, were given by appropriate schedules before two types of trainings and the changes in number of SOM mRNA positive neurons were examined in hippocampus and cerebral cortex. The results showed that: (1) when compared with non-trained control group, the number of SOM mRNA positive neurons were significantly increased in these two brain regions in rats that had been trained with either of two trainings; (2) the increase of SOM mRNA positive neurons in both regions following behavioral trainings could be significantly blocked by i.c.v. injection of NAME, and the injection also impaired the learning and memory formation as compared to that in control groups with trainings only. These data indicate that endogenous NO is critical for the formation of learning and memory, and also for the enhanced SOM mRNA expression that accompanies relevant behavioral trainings. It is proposed that NO might be involved in the cascade of central neurochemical changes, including the induction of somatostatin synthesis in related brain areas that underlie the learning and memory processes.