CAJ | 학술논문

通过细胞外记录方法记录场兴奋性突触后电位(field excitatory postsynaptic potential,fEPSP)的变化是研究突触可塑性,诸如长时程增强(long-term potentiation,LTP)和双脉冲可塑性(paired.pulse plasticity,PPP)的最常见方法之一.fEPSP波形的起始斜率、起始面积、峰值及总面积等的变化常用作判断突触可塑性增强或减弱的标准.在相同记录结果中测量fEPSP波形不同部位通常会有不同的结果,因此可能得出不同的结论,这些往往会被研究者忽略.本文通过测量小鼠海马CAl区细胞fEPSP波形的起始斜率、起始面积、峰值、总面积及时间参数等,分析比较高频刺激(high-frequency stimulation,HFS)诱发的突触可塑性,包括LTP和PPP的变化.结果显示,LTP过程中AMPA受体动力学变化加快,且在同一记录中,fEPSP波形不同部位的测鼍分析可以产生较大幅度的LTP和PPP差异.给予HFS后,双脉冲诱发fEPSP的比率在测量起始面积时略有下降,但在测量起始斜率时则显著增加,这些结果可能导致相反的结论.因此,全面仔细地分析fEPSP波形在整个实验中的变化对正确了解突触可塑性至关重要.
통과세포외기록방법기록장흥강성돌촉후전위(field excitatory postsynaptic potential,fEPSP)적변화시연구돌촉가소성,제여장시정증강(long-term potentiation,LTP)화쌍맥충가소성(paired.pulse plasticity,PPP)적최상견방법지일.fEPSP파형적기시사솔、기시면적、봉치급총면적등적변화상용작판단돌촉가소성증강혹감약적표준.재상동기록결과중측량fEPSP파형불동부위통상회유불동적결과,인차가능득출불동적결론,저사왕왕회피연구자홀략.본문통과측량소서해마CAl구세포fEPSP파형적기시사솔、기시면적、봉치、총면적급시간삼수등,분석비교고빈자격(high-frequency stimulation,HFS)유발적돌촉가소성,포괄LTP화PPP적변화.결과현시,LTP과정중AMPA수체동역학변화가쾌,차재동일기록중,fEPSP파형불동부위적측타분석가이산생교대폭도적LTP화PPP차이.급여HFS후,쌍맥충유발fEPSP적비솔재측량기시면적시략유하강,단재측량기시사솔시칙현저증가,저사결과가능도치상반적결론.인차,전면자세지분석fEPSP파형재정개실험중적변화대정학료해돌촉가소성지관중요.
Extracellular recordings of field excitatory postsynaptic potential (fEPSP) is one of the most common ways for studies ofsynaptic plasticity, such as long-term potentiation (LTP) and paired-pulse plasticity (PPP). The measurement of the changes in thedifferent components of fEPSP waveform, such as the initial slope, initial area, peak amplitude and whole area, were commonly usedas criteria for the judgement of potentiation or depression of synaptic plasticity. However, the differences in the conclusions drawnfrom measuring different components of fEPSP waveform at the same recording have still been largely ignored. Here we compared high-frequency stimulation (HFS)-evoked synaptic plasticity, both LTP and PPP, by measuring different components of fEPSP waveform,including the initial slope, initial area, peak amplitude, whole area and time course. The results not only indicated the acceleration of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor kinetics underlies LTP in hippocampal CAI region of mice,but also showed that different measurements of fEPSP waveform at the same recording'result in different magnitudes of LTP anddifferent forms of PPP in hippocampal CAl region of mice. After HFS, the paired-pulse ratio was slightly decreased by measurementof the initial area, but obviously increased by measurement of the initial slope of the pair fEPSPs. These results might draw apparentlycontradictory conclusions. Therefore, careful and complete analysis of the data from different parts of fEPSP waveforms is importantfor reflection of the faithful changes in synaptic plasticity.