CAJ | 학술논문

目的:探讨肝X受体(LXR)、11β-羟基类固醇脱氢酶1(11β-HSD1)和糖皮质激素受体(GR)在糖尿病发生、发展中的作用和意义.方法:应用链佐脲菌素(STZ)诱导建立糖尿病大鼠模型,将止常大鼠作为对照组.应用实时定量-聚合酶链反应(real-time PCR)的方法测定2组大鼠肝组织内LXR mRNA、11β-HSD1 mRNA和GR mRNA的表达.结果:糖尿病组大鼠肝组织内LXR mRNA较对照组明显升高(P<0.01),11β-HSD1 mRNA较对照组明显降低(P<0.05),GR mRNA较对照组有所下降.但尚未达到统计学意义.结论:糖尿病大鼠肝内LXR水平呈代偿性升高,伴局部组织糖皮质激素作用减弱,这可能为机体对高糖应激的一种代偿性的自身调节机制.
목적:탐토간X수체(LXR)、11β-간기류고순탈경매1(11β-HSD1)화당피질격소수체(GR)재당뇨병발생、발전중적작용화의의.방법:응용련좌뇨균소(STZ)유도건립당뇨병대서모형,장지상대서작위대조조.응용실시정량-취합매련반응(real-time PCR)적방법측정2조대서간조직내LXR mRNA、11β-HSD1 mRNA화GR mRNA적표체.결과:당뇨병조대서간조직내LXR mRNA교대조조명현승고(P<0.01),11β-HSD1 mRNA교대조조명현강저(P<0.05),GR mRNA교대조조유소하강.단상미체도통계학의의.결론:당뇨병대서간내LXR수평정대상성승고,반국부조직당피질격소작용감약,저가능위궤체대고당응격적일충대상성적자신조절궤제.
Objective To investigate the role and significance of liver X receptor (LXR), 11 hydroxysteroid dehydrogenase 1 (11β-HSDI) and glucocorticoid receptor (GR) in the development of rat diabetes models. Methods Twelve male Sprague-Dawley (SD) rats were randomly divided into control group and streptozotocin(STZ)-induced diabetes group. The mRNA levels of LXR, 11β-HSD1 and GR in the liver were determined by real-time quantitative polymerase chain reaction. Results The mRNA level of LXR more increased in diabetes mellitus (DM) group than that in control group(P<0.01), and the mRNA level of 11β-HSD1 more decreased in DM group than that in control group(P<0.05). The expression of GR mRNA also decreased in DM group, yet was not statistically significant. Conclusions The level of LXR increase compensatively in DM rats, accompanied by a decreased roll of glucocorticoid in local tissue. It might be a self-regulation mechanism to hyperglycemic stress in body.