CAJ | 학술논문

为研究E2F3转录因子的结构、功能及其与肿瘤发生的联系,从人组织中克隆E213基因,通过巢氏PCR和桥联PCR成功构建了E2f3的原核表达载体,实现了对E2P3基因的原核表达,并用Western检测了重组蛋白.研究中发现E2f3 cDNA编码序列中含有一段富含GC的区域.该区域对E2f3基因的PCR扩增影响很大.
위연구E2F3전록인자적결구、공능급기여종류발생적련계,종인조직중극륭E213기인,통과소씨PCR화교련PCR성공구건료E2f3적원핵표체재체,실현료대E2P3기인적원핵표체,병용Western검측료중조단백.연구중발현E2f3 cDNA편마서렬중함유일단부함GC적구역.해구역대E2f3기인적PCR확증영향흔대.
E2F3, a transcription factor, in order to study its structure, function and relationship with the occurrence of tumor, E2F3 gene from human tissue were cloned, constructed a prokaryotic expression vector by nested and o-verlap PCR to express E2F3. The full-length of E2F3 gene has been successfully cloned and recombinant protein expressed was tested by Western blotting. The result show that there is a length of GC-rich region in E2F3 cDNA which greatly affected the amplification of E2F3 gene.