CAJ | 학술논문

通过对一株新分离到的耐盐原油降解菌进行形态学观察和生理生化鉴定,确定该菌株为假单胞菌(Pseudomonas sp).并对其生长特性和降解特性做了初步研究,确定了该菌株降解原油的最适条件为:原油的质量浓度为0.5 g/L、NaCl的质量浓度为30 g/L、pH值为8、温度30℃、摇床转速140 r/min、接种量为3%.在最适条件下,经过5 d的培养,原油的降解率为68%.实验结果表明该菌株治理海洋石油污染的应用前景较大.
통과대일주신분리도적내염원유강해균진행형태학관찰화생리생화감정,학정해균주위가단포균(Pseudomonas sp).병대기생장특성화강해특성주료초보연구,학정료해균주강해원유적최괄조건위:원유적질량농도위0.5 g/L、NaCl적질량농도위30 g/L、pH치위8、온도30℃、요상전속140 r/min、접충량위3%.재최괄조건하,경과5 d적배양,원유적강해솔위68%.실험결과표명해균주치리해양석유오염적응용전경교대.
A strain that has a capability to degrade crude oil was isolated from the extracted oil waste water. The strain could use crude oil as a sole carbon source. It was primarily identified as pseudomonas sp based on the morphological and physio-biochemical characteristics. Under the optimal growth conditions,with substrate concentration of 0.5 g/L,density of NaCl 30 g/L,pH of 8,temperature of 30℃,rotating rate of 140 r/min,and inoculation amount of 3%. The crude oil could be degraded in the high efficiency,more than 68%. The result of experiment showed that the strain could be used in the bioremediation of marine oil pollution.