CAJ | 학술논문

目的研究应用吡喹酮后小鼠肺部血吸虫卵肉芽肿内细胞的变化.方法 48只小鼠均分4组.A组:虫卵致敏组,先于小鼠腹壁皮下注入新鲜日本血吸虫卵,10 d后由尾静脉注入虫卵;B组:吡喹酮短期应用组,致敏方法同A组,于尾静脉注入虫卵前1 d起灌服吡喹酮300 mg/(kg·d)连续3 d;C组:吡喹酮持续应用组,致敏方法同A组,于尾静脉注入虫卵前1 d起灌服吡喹酮75 mg/kg,1日2次,连续5 d,停药2 d后反复用药至剖检;D组:第29天起应用吡喹酮组,服法同C组.各组均在尾静脉注射虫卵后7、14、28、56 d分别剖杀3只,取肺组织切片,HE染色,在每组肺连续切片内选含毛蚴的虫卵肉芽肿25～30个,分类计数每个肉芽肿内的中性粒细胞、嗜酸性粒细胞、淋巴细胞、纤维母细胞、巨噬细胞,计算其平均值并作比较.结果与A组比较,应用吡喹酮的3个实验组,中性粒细胞、嗜酸性粒细胞、巨噬细胞均显著减少,纤维母细胞增高的幅度也明显为低,差异均有统计学意义,而以C组最为显著,第56天,中性粒细胞、嗜酸性粒细胞、巨噬细胞分别减少了54.4%、87.0%和23.1%,纤维母细胞增高的幅度减少了59.4%.各组淋巴细胞数目变化不大.结论吡喹酮能明显抑制虫卵肉芽肿内炎细胞的聚集、纤维母细胞的增生.
목적 연구응용필규동후소서폐부혈흡충란육아종내세포적변화.방법 48지소서균분4조.A조:충란치민조,선우소서복벽피하주입신선일본혈흡충란,10 d후유미정맥주입충란;B조:필규동단기응용조,치민방법동A조,우미정맥주입충란전1 d기관복필규동300 mg/(kg·d)련속3 d;C조:필규동지속응용조,치민방법동A조,우미정맥주입충란전1 d기관복필규동75 mg/kg,1일2차,련속5 d,정약2 d후반복용약지부검;D조:제29천기응용필규동조,복법동C조.각조균재미정맥주사충란후7、14、28、56 d분별부살3지,취폐조직절편,HE염색,재매조폐련속절편내선함모유적충란육아종25～30개,분류계수매개육아종내적중성립세포、기산성립세포、림파세포、섬유모세포、거서세포,계산기평균치병작비교.결과 여A조비교,응용필규동적3개실험조,중성립세포、기산성립세포、거서세포균현저감소,섬유모세포증고적폭도야명현위저,차이균유통계학의의,이이C조최위현저,제56천,중성립세포、기산성립세포、거서세포분별감소료54.4%、87.0%화23.1%,섬유모세포증고적폭도감소료59.4%.각조림파세포수목변화불대.결론 필규동능명현억제충란육아종내염세포적취집、섬유모세포적증생.
Objective To study the effect of praziquantel on cells within sehistosomal ovum granuloma in the lung of mice.Methods Forty-eight mice were divided into 4 groups.Group A:first,the mice were injected with sehistosomal ova hypodermically in abdomen,and 10 days later,injected with schistosomal ova intravenously in the cauda;Group B:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel[300 mg/(kg·d)]for 3 days,one day before the intravenous injection of the ova;Group C:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel(75 mg/kg,B.i.d.) for 5 days weekly until the mice were sacrificed;Group D:the same as Group C but praziquantel was given to the mice from the 29th after the intravenous injection of the ova.Three mice of each group were sacrificed on the 7th,14th,28th,56th day after the intravenous injection of the ova and the lung tissues were fixed with formalin and the slices were HE stained.Twenty-five to thirty pieces of schistosomal ovum granuloma were examined and the neutrophilic granulocytes,eosinocytes,lymphocytes,fibroblasts and macrophages within the ovum granulomas were counted and the mean numbers of them of each group were calculated and compared.Results Compared with Group A,the mean numbers of neutrophilic granulocytes,eosinocytes and macrophages within the ovum granulomas were decreased significantly,and the extend of the increase of fibroblasts reduced significantly in the three groups administered with praziquantel,and especially in Group C.On the 56th day after the intravenous injection of the ova,the mean numbers of neutrophilic granulocytes,eosinocytes and macrophages decreased by 54.4%、87.0% and 23.1%,and the extend of the increase of fibroblasts reduce by 59.4%,respectively in Group C,compared with Group A.The numbers of lymphocytes did not change very much in 4 groups.Conclusion Praziquantel can restrain inflammatory cells and the hyperplasia of fibroblasts within schistosomal ovum granulomas.