CAJ | 학술논문

为揭示南方红豆杉种子内源激素与休眠的关系,采用酶联免疫吸附法(ELISA)测定了经过层积处理的种皮和胚乳的脱落酸(ABA)、赤霉素(GA3 )、吲哚乙酸(IAA)、玉米素核苷(ZR)4种内源激素含量的变化情况.结果表明:种子胚乳中内源ABA的含量随着层积时间的延长而逐渐下降,GA含量增加,IAA和ZR的含量先增加后降低,GA/ABA、IAA/ABA和ZR/ABA逐渐增大,休眠随之解除.种皮中内源ABA、GA、IAA和ZR的含量均随着层积时间的延长而逐渐下降.其中GA/ ABA的变化较大,因此,南方红豆杉种子休眠的限制因子很可能是ABA和GA的平衡调控作用.
위게시남방홍두삼충자내원격소여휴면적관계,채용매련면역흡부법(ELISA)측정료경과층적처리적충피화배유적탈락산(ABA)、적매소(GA3 )、신타을산(IAA)、옥미소핵감(ZR)4충내원격소함량적변화정황.결과표명:충자배유중내원ABA적함량수착층적시간적연장이축점하강,GA함량증가,IAA화ZR적함량선증가후강저,GA/ABA、IAA/ABA화ZR/ABA축점증대,휴면수지해제.충피중내원ABA、GA、IAA화ZR적함량균수착층적시간적연장이축점하강.기중GA/ ABA적변화교대,인차,남방홍두삼충자휴면적한제인자흔가능시ABA화GA적평형조공작용.
In order to characterize relationship between endogenous phytohormones and dormancy of Taxus chinensis var.mairei,changes in contents of four kinds of endogenous phytohormones,i.e.,abscisic acid(ABA),gibberellin(GA3),indole acetic acid(IAA)and zeatin riboside(ZR)in spermoderm and endosperm of Taxus mairei were analysed by enzyme-linked immunosorbent assays(ELISA). The results showed that ABA contents were gradually decreased in endosperm during stratification,while GA contents were increased and IAA and ZR contents firstly increased and then decreased. Calculated ratio data of GA/ABA,IAA/ABA and ZR/ABA exhibited gradually increasing trends within stratification time and dormancy was relieved accordingly with them. In spermoderm,endogenous ABA,GA,IAA and ZR contents were gradually decreased through stratification. The change in GA/ABA ratio was greater than the other two ones. So it was suggested that the tradeoff between ABA and GA contents could control the dormancy process in T.chinensis var.mairei seed.