国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
2期
114-117
,共4页
王荣国%章永东%周卫%李莉%张万强
王榮國%章永東%週衛%李莉%張萬彊
왕영국%장영동%주위%리리%장만강
三七凝胶%黏连%椎板切除术%TIMP-1%MMP-1
三七凝膠%黏連%椎闆切除術%TIMP-1%MMP-1
삼칠응효%점련%추판절제술%TIMP-1%MMP-1
Sanchi Gel%Adhesion%Laminectomy%Inhibitor of metalloproteinase-1%Matrix metalloproteinase- 1
目的 探讨三七凝胶对椎板切除术后硬膜外黏连基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶抑制剂-1(TIMP-1)表达的影响.方法 建立椎板切除术模型,将72只大鼠按随机数字表法分为生理盐水组、黏连平组、凝胶组和三七凝胶组,各18只,分别在术后第7、14、21天采用免疫组化进行动态观察,分析愈合过程中TIMP-1和MMP-1的表达.结果 不同处理方法对TIMP-1、MMP-1的影响作用不同,作用强度存在差异.术后14 d、21 d时TIMP-1的表达逐渐增强,但阳性细胞数逐渐减少,三七凝胶组的TIMP-1表达均弱于其他三组(P均＜0.05);术后14 d各组MMP-1的表达开始增强,但无明显差异(P＞0.05);术后21 d三七凝胶组MMP-1的表达强于其他三组(P＜0.05),各组中MMP-1的阳性细胞数随时间的延长逐渐减少.结论 三七凝胶预防硬膜外黏连的作用可能与调控TIMP-1和MMP-1的表达有关.
目的 探討三七凝膠對椎闆切除術後硬膜外黏連基質金屬蛋白酶-1(MMP-1)、基質金屬蛋白酶抑製劑-1(TIMP-1)錶達的影響.方法 建立椎闆切除術模型,將72隻大鼠按隨機數字錶法分為生理鹽水組、黏連平組、凝膠組和三七凝膠組,各18隻,分彆在術後第7、14、21天採用免疫組化進行動態觀察,分析愈閤過程中TIMP-1和MMP-1的錶達.結果 不同處理方法對TIMP-1、MMP-1的影響作用不同,作用彊度存在差異.術後14 d、21 d時TIMP-1的錶達逐漸增彊,但暘性細胞數逐漸減少,三七凝膠組的TIMP-1錶達均弱于其他三組(P均＜0.05);術後14 d各組MMP-1的錶達開始增彊,但無明顯差異(P＞0.05);術後21 d三七凝膠組MMP-1的錶達彊于其他三組(P＜0.05),各組中MMP-1的暘性細胞數隨時間的延長逐漸減少.結論 三七凝膠預防硬膜外黏連的作用可能與調控TIMP-1和MMP-1的錶達有關.
목적 탐토삼칠응효대추판절제술후경막외점련기질금속단백매-1(MMP-1)、기질금속단백매억제제-1(TIMP-1)표체적영향.방법 건립추판절제술모형,장72지대서안수궤수자표법분위생리염수조、점련평조、응효조화삼칠응효조,각18지,분별재술후제7、14、21천채용면역조화진행동태관찰,분석유합과정중TIMP-1화MMP-1적표체.결과 불동처리방법대TIMP-1、MMP-1적영향작용불동,작용강도존재차이.술후14 d、21 d시TIMP-1적표체축점증강,단양성세포수축점감소,삼칠응효조적TIMP-1표체균약우기타삼조(P균＜0.05);술후14 d각조MMP-1적표체개시증강,단무명현차이(P＞0.05);술후21 d삼칠응효조MMP-1적표체강우기타삼조(P＜0.05),각조중MMP-1적양성세포수수시간적연장축점감소.결론 삼칠응효예방경막외점련적작용가능여조공TIMP-1화MMP-1적표체유관.
Objective To explore the effects of Sanchi gel on MMP-1 and TIMP-1 expression in epidural adhesion after laminectomy. Methods Laminectomy model was set up in SD rats. 72 SD model rats were divided randomly into Saline group, Zhanlp group, Carbopol Gel group and Sanchi Gel group, with 18 rats in each group. On 7, 14 and 21 days after the laminectomy, immunohistochemistry (method of S-P) was adopted to detect the expression of TIMP-1 and MMP-1. Results Different treatments had different influence on TIMP-1 and MMP-1 expression in epidural adhesion. The expression of TIMP-1 increased gradually on 14 dand 21 d after operation, and Sanqi Gel group showed weaker expression than the other groups (P＜0.05), but the number of positive cells decreased gradually. As to the expression of MMP-1, there was no difference among each group at the end of the second week (P＞0.05). There was a difference between Sanchi Gel group and the other groups at the end of third week (21 d) (P＜0.05) after operation. Sanchi Gel group showed higher expression of TIMP-1 than the other groups. The number of positive cells sharply decreased from 14 d to 21 d after operation. Conclusion Sanchi Gel has a significant preventive effect on fibrous scar formation after laminectomy. The possible mechanism of preventing epidural adhesion after laminectomy by Sanchi Gel could be its regulating and controlling the expression of TIMP-1 and MMP-1 in epidural tissue.
