CAJ | 학술논문

目的研究应力环境对普通组织培养基体外保存的骨软骨移植物的保存质量的影响,从而改进组织培养法保存软骨的效果.方法用摇床作为动态加载装置对体外保存的骨软骨移植物进行持续周期性应力加载,以模拟体内关节软骨的应力环境.摇床在37℃的孵箱中工作,频率设置为1 Hz.在储存2周时,用Western bloting的方法对关节软骨中的肌动蛋白β-actin进行半定量分析,用透射电镜观察胶原的超微结构,并与对照组比较.结果与静态环境下保存相比,动态加载环境使关节软骨细胞外基质中β-actin的表达水平明显提高,并能更好地保护基质中胶原成分的超微结构.结论应力环境有利于保存人同种异体移植物软骨细胞和胶原的超微结构.动态力学加载可提供一种更好的库存人同种异体骨软骨移植物的方法.
목적 연구응력배경대보통조직배양기체외보존적골연골이식물적보존질량적영향,종이개진조직배양법보존연골적효과.방법용요상작위동태가재장치대체외보존적골연골이식물진행지속주기성응력가재,이모의체내관절연골적응력배경.요상재37℃적부상중공작,빈솔설치위1 Hz.재저존2주시,용Western bloting적방법대관절연골중적기동단백β-actin진행반정량분석,용투사전경관찰효원적초미결구,병여대조조비교.결과여정태배경하보존상비,동태가재배경사관절연골세포외기질중β-actin적표체수평명현제고,병능경호지보호기질중효원성분적초미결구.결론응력배경유리우보존인동충이체이식물연골세포화효원적초미결구.동태역학가재가제공일충경호적고존인동충이체골연골이식물적방법.
Objective To study the effect of the loading environment on the chondrocytes and the collagen ultrastructure at articular cartilage of the osteochondral allograft graft preserved by tissue culture method so as to improve the preservation effect. Methods A kind of shaking table was used as a dynamic loading device for storage of the osteochondral allograft to simulate the mechanical environment of normal articular cartilage in vivo. The device worked in an incubator at 3711 and the frequency was 1Hz. Western blotting was used for analysis of β-actin at the articular cartilage of the graft for investigating the effects of stress condition on the chondrocyte function. Transmission electron microscopy was used to observe the collagen ultrastructure at the articular cartilage of the graft two weeks after storage and compared with the control group. Results The dynamic loading condition could significantly increase the expression of β-actin and keep the ultrastructure of the collagen fibril in articular cartilage compared with the static condition. Conclusions The loading condition benefits the chondrocyte function and collagen ultrastructure of the articular cartilage in human osteochondral allograft. The dynamic mechanical loading may provide a better method in preservation of the human osteochondral allograft.