中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
1期
24-27
,共4页
唐亮%阮艳君%张巡淼%孙道远
唐亮%阮豔君%張巡淼%孫道遠
당량%원염군%장순묘%손도원
二氧化氮%肺水肿%大鼠%模型,动物
二氧化氮%肺水腫%大鼠%模型,動物
이양화담%폐수종%대서%모형,동물
Nitrogen dioxide%Pulmonary edema%Rats%Model,animal
目的 建立高浓度二氧化氮(NO2)染毒SD大鼠致急性肺水肿的模型.方法 将SD大鼠随机分为对照组(8只)和染毒组(30只),染毒组以(6747.47±215.24)mg/m3 NO2通过动式染毒柜染毒90 s后以净空气平衡换气30 min;对照组大鼠入染毒柜以净空气平衡30 min.染毒组分别在6、12、18、24 h拍摄大鼠胸片,各时间点取6只大鼠留取血样后处死,取肺组织做病理检查,进行肺湿/干系数测定.检测全血红细胞膜超氧化物歧化酶(SOD)活力和血浆中心钠素(ANP)浓度.结果 染毒组大鼠均在24 h内发生肺水肿,胸片显示,斑片云雾状阴影,12 h时加重,18 h时斑片影加深,遍及全肺,呈现"白肺"状,24 h后无明显恢复.HE染色显示,染毒组6 h肺泡间隙增大,泡内有少量嗜伊红液体渗出,12 h后肺泡融合,泡内嗜伊红液体增多,18 h后肺泡及组织中充满嗜伊红液体,至24 h时无恢复.染毒组6、12、18、24 h肺湿/干系数分别为5.60±0.20、6.89±0.25、8.03±0.47、7.81±0.45,与对照组(4.72±0.06)比较,差异均有统计学意义(P＜0.01);染毒组12、18、24 h肺湿/干系数与6 h相比,差异均有统计学意义(P＜0.01);染毒后18、24 h肺湿/干系数与12 h相比,差异有统计学意义(P＜0.01).与对照组比较,染毒组6、12、18、24 h红细胞膜SOD活力均明显降低,差异有统计学意义(P＜0.01).染毒组18、24 h血浆中ANP浓度分别为(136.66±35.37)、(134.10±60.41)ng/ml,明显高于对照组[(31.31±13.06)ng/ml]、染毒组6、12 h[分别为(34.71±13.42)、(47.98±7.86)ng/ml],差异均有统计学意义(P＜0.01).结论 高浓度NO2以动式染毒柜染毒SD大鼠复制急性肺水肿模型是可行的.
目的 建立高濃度二氧化氮(NO2)染毒SD大鼠緻急性肺水腫的模型.方法 將SD大鼠隨機分為對照組(8隻)和染毒組(30隻),染毒組以(6747.47±215.24)mg/m3 NO2通過動式染毒櫃染毒90 s後以淨空氣平衡換氣30 min;對照組大鼠入染毒櫃以淨空氣平衡30 min.染毒組分彆在6、12、18、24 h拍攝大鼠胸片,各時間點取6隻大鼠留取血樣後處死,取肺組織做病理檢查,進行肺濕/榦繫數測定.檢測全血紅細胞膜超氧化物歧化酶(SOD)活力和血漿中心鈉素(ANP)濃度.結果 染毒組大鼠均在24 h內髮生肺水腫,胸片顯示,斑片雲霧狀陰影,12 h時加重,18 h時斑片影加深,遍及全肺,呈現"白肺"狀,24 h後無明顯恢複.HE染色顯示,染毒組6 h肺泡間隙增大,泡內有少量嗜伊紅液體滲齣,12 h後肺泡融閤,泡內嗜伊紅液體增多,18 h後肺泡及組織中充滿嗜伊紅液體,至24 h時無恢複.染毒組6、12、18、24 h肺濕/榦繫數分彆為5.60±0.20、6.89±0.25、8.03±0.47、7.81±0.45,與對照組(4.72±0.06)比較,差異均有統計學意義(P＜0.01);染毒組12、18、24 h肺濕/榦繫數與6 h相比,差異均有統計學意義(P＜0.01);染毒後18、24 h肺濕/榦繫數與12 h相比,差異有統計學意義(P＜0.01).與對照組比較,染毒組6、12、18、24 h紅細胞膜SOD活力均明顯降低,差異有統計學意義(P＜0.01).染毒組18、24 h血漿中ANP濃度分彆為(136.66±35.37)、(134.10±60.41)ng/ml,明顯高于對照組[(31.31±13.06)ng/ml]、染毒組6、12 h[分彆為(34.71±13.42)、(47.98±7.86)ng/ml],差異均有統計學意義(P＜0.01).結論 高濃度NO2以動式染毒櫃染毒SD大鼠複製急性肺水腫模型是可行的.
목적 건립고농도이양화담(NO2)염독SD대서치급성폐수종적모형.방법 장SD대서수궤분위대조조(8지)화염독조(30지),염독조이(6747.47±215.24)mg/m3 NO2통과동식염독거염독90 s후이정공기평형환기30 min;대조조대서입염독거이정공기평형30 min.염독조분별재6、12、18、24 h박섭대서흉편,각시간점취6지대서류취혈양후처사,취폐조직주병리검사,진행폐습/간계수측정.검측전혈홍세포막초양화물기화매(SOD)활력화혈장중심납소(ANP)농도.결과 염독조대서균재24 h내발생폐수종,흉편현시,반편운무상음영,12 h시가중,18 h시반편영가심,편급전폐,정현"백폐"상,24 h후무명현회복.HE염색현시,염독조6 h폐포간극증대,포내유소량기이홍액체삼출,12 h후폐포융합,포내기이홍액체증다,18 h후폐포급조직중충만기이홍액체,지24 h시무회복.염독조6、12、18、24 h폐습/간계수분별위5.60±0.20、6.89±0.25、8.03±0.47、7.81±0.45,여대조조(4.72±0.06)비교,차이균유통계학의의(P＜0.01);염독조12、18、24 h폐습/간계수여6 h상비,차이균유통계학의의(P＜0.01);염독후18、24 h폐습/간계수여12 h상비,차이유통계학의의(P＜0.01).여대조조비교,염독조6、12、18、24 h홍세포막SOD활력균명현강저,차이유통계학의의(P＜0.01).염독조18、24 h혈장중ANP농도분별위(136.66±35.37)、(134.10±60.41)ng/ml,명현고우대조조[(31.31±13.06)ng/ml]、염독조6、12 h[분별위(34.71±13.42)、(47.98±7.86)ng/ml],차이균유통계학의의(P＜0.01).결론 고농도NO2이동식염독거염독SD대서복제급성폐수종모형시가행적.
Objective To establish the rats model of acute pulmonary edema induced by inhalation of high concentrations of nitrogen dioxide (NO2). Methods 38 SD rats were divided into the experimental group ( n=30 ) and the control group ( n=8 ). 30 rats in the experimental group were exposed to (6747.47±2 5.24) mg/m3 NO2 in the exposure system. At the time point of 6, 12, 18, 24 h, chest X-ray examination was taken for the experimental group And at each time point, 6 rats were sacrificed after taking blood samples. After sacrificing,the lung of rats was taken for pathological examination and calculated lung wet/dry weight ratio. Erythrocyte superoxide dismutase (SOD) activity and plasma atrial natriuretic peptide (ANP) concentration of blood samples were detected. Results Acute pulmonary edema was successfully induced by exposure to NO2 in 30 rats within 24 hours. There were some cloudy shadows without clear edge on the chest X-ray. To the time point of 12 hours, shadows combined with each other, and to the time point of 18 hours, the whole lung became "white"on the X-ray. The situation stabilized but not improved at the time point of 24 hours. HE staining of the lung tissue showed that to the time point of 6 hours, the alveolar gap increased and small amount of eosinophilic liquid leaked into alveolar. To the time point of 12 hours, alveolar combined with each other and eosinophilic liquid increased in amount. To the time point of 18 hours, the whole alveolar was filled with eosinophilic liquid and the situation stabilized till the time point of 24 hours. Wet/dry weight ratio of the experimental group at each time point were 5.6±0.20、6.89±0.25、8.03±-0.47、7.81 ± 0.45. There was significant difference compared with the control group which was 4.72 ± 0.06 (P＜0.01). There was statistical difference between 12,18,24 h and 6 h time points (P＜0.01). Moreover, statistical difference was observed between 18,24 h and 12 h time points for wet/dry weight ratio (P＜0.01). The erythrocvte SOD activity reduced significantly. Compared with the control group, there was a statistical difference (P＜0.01) at each time point. After exposure of 18 and 24hours, plasma A NP concentration ( 136.66± 35.37) and ( 134.10±60.41 ) ng/ml respectively, which were higher than (31.31 ± 13.06 ) ng/ml of control group and ( 34.71 ± 13.42 ) ng/ml of 6 hours time point and (47.98 ± 7.86 )ng/ml. The differences were significant (P＜0.01),Conclusion High concentrations of NO2 can induce acute pulmonary edema model successfully in SD rats.
