CAJ | 학술논문

目的研究2,5-己二酮(2,5-HD)对小鼠视网膜组织形态学的影响及对视网膜组织的脂质过氧化作用,揭示正己烷对视网膜损伤的发病机制.方法 48只昆明种小鼠随机分为空白对照组、阴性对照组和2,5-HD染毒组.空白对照组12只,不做任何处理；阴性对照组12只,腹腔注射生理盐水；2,5-HD染毒组24只(分为2、4和8周染毒组),腹腔注射质量分数为2.5％的2,5-H-HD溶液,剂量为400 mg/kg,每日给药1次.光学显微镜下观察2,5-HD对视网膜组织的病理形态学影响；并且测定视网膜组织匀浆中超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量.结果空白对照及阴性对照组小鼠视网膜结构正常.2,5-HD染毒8周组光感受器内外节分界不清,排列疏松紊乱；外丛状层呈疏松网状结构,染色不均；神经节细胞层可见胞核深染固缩坏死.随着2,5-HD染毒时间的延长,SOD活力逐渐降低,MDA含量增加,组间比较差异有统计学意义(P＜0.05).结论 2,5-HD可造成小鼠视网膜组织损伤,脂质过氧化作用是致视网膜损伤的机制之一.
목적 연구2,5-기이동(2,5-HD)대소서시망막조직형태학적영향급대시망막조직적지질과양화작용,게시정기완대시망막손상적발병궤제.방법 48지곤명충소서수궤분위공백대조조、음성대조조화2,5-HD염독조.공백대조조12지,불주임하처리；음성대조조12지,복강주사생리염수；2,5-HD염독조24지(분위2、4화8주염독조),복강주사질량분수위2.5％적2,5-H-HD용액,제량위400 mg/kg,매일급약1차.광학현미경하관찰2,5-HD대시망막조직적병리형태학영향；병차측정시망막조직균장중초양화물기화매(SOD)활력급병이철(MDA)함량.결과 공백대조급음성대조조소서시망막결구정상.2,5-HD염독8주조광감수기내외절분계불청,배렬소송문란；외총상층정소송망상결구,염색불균；신경절세포층가견포핵심염고축배사.수착2,5-HD염독시간적연장,SOD활력축점강저,MDA함량증가,조간비교차이유통계학의의(P＜0.05).결론 2,5-HD가조성소서시망막조직손상,지질과양화작용시치시망막손상적궤제지일.
Objective To study toxic effects of 2,5-hexanedione (2,5-HD) on pathology and lipid peroxidation in mouse retina.Methods Forty-eight mice were randomly divided into blank control group ( 12mice),negative control group exposed to normal solution ( 12 mice) and group exposed to 2,5-HD for 2.4 and 8 weeks,respectively (24 mice) by intraperitoneal injection (2.5％ 2,5-HD) at the dose of 400 mg/kg.The pathological changes of mouse retina were examined undcr light microscope.The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in mouse retina were detected.Results The retinal structure in the blank and negative control groups was normal.In mice exposed to 2,5-HD for 8 weeks,the swelling of outer and inner segments and disorder arrangement of the segments without clear boundary were found.The staining of outer plexiform layers was uneven and the irregular loose structure appeared.The hyperchromatic pyknotic and necrosis nuclei were presented in ganglion cells layer.Compared with the control and blank groups,the activities of SOD gradually and significantly reduced and the concentrations of MDA increased in group exposed to 2,5-HD (P＜0.05).Conclusion 2,5-HD can induce the injury of retina tissues of mice,which may be associated with the lipid peroxidation.