CAJ | 학술논문

目的了解我国不同疟疾流行区间日疟原虫种群结构和遗传多样性特征,积累我国间日疟原虫遗传相关数据.方法收集云南、海南、河南流行区间日疟患者血样,血涂片鉴定间日疟原虫阳性者抽提血液基因组,采用巢式/半巢氏PCR方法扩增特异性2.21微卫星片段,对扩增阳性产物进行基因扫描检测,根据检测微卫星的重复序列进行STR分型,并应用GENALEX软件计算等位基因频率、等位基因数目以及期望杂合度(expected heterozygosity,He).结果间日疟原虫2.21微卫星在不同地区间日疟原虫样本中呈现高度的多态性,其等位基因数目变化范围为4～9,期望杂合度为0.613～0.853,比较不同地区,云南地区间日疟原虫等位基因数目为9,期望杂合度为0.853,变异度最高.结论我国不同地区间日疟原虫基因组具有较高的遗传多样性,其种群结构具有地区特征,这可能与各种群传疟媒介种类不同及地理环境差异等相关.
목적 료해아국불동학질류행구간일학원충충군결구화유전다양성특정,적루아국간일학원충유전상관수거.방법 수집운남、해남、하남류행구간일학환자혈양,혈도편감정간일학원충양성자추제혈액기인조,채용소식/반소씨PCR방법확증특이성2.21미위성편단,대확증양성산물진행기인소묘검측,근거검측미위성적중복서렬진행STR분형,병응용GENALEX연건계산등위기인빈솔、등위기인수목이급기망잡합도(expected heterozygosity,He).결과 간일학원충2.21미위성재불동지구간일학원충양본중정현고도적다태성,기등위기인수목변화범위위4～9,기망잡합도위0.613～0.853,비교불동지구,운남지구간일학원충등위기인수목위9,기망잡합도위0.853,변이도최고.결론아국불동지구간일학원충기인조구유교고적유전다양성,기충군결구구유지구특정,저가능여각충군전학매개충류불동급지리배경차이등상관.
Objective To investigate the population structure and genetic diversity of Plasmodium vivax isolates from malaria-endemic areas in China,and to set up genetic database of vivax malaria.Methods Blood samples of symptomatic patients that were positive for P. vivax by microscope were collected from Yunnan,Hainan and Henan provinces and used for nested PCR amplification of DNA fragment containing microsatellite loci and detection of the microsatellite by Genescan.Then GENALEX software was used to calculate haplotype frequency,number of alleles and expeted heterozygosity ( He ).Results The length of 2.21 microsatellite was highly variable in P.vivax isolates from different areas in China.The number of the microsatellite alleles varied from 4 to 9,and expected heterozygosity ( He ) was 0.613 ～ 0.853.The microsatellite showed the highest diversity in the isolates from Yunnan where the number of allele is 9 and He means 0.853 compared to those in other areas.Conclusion The P.vivax isolates from different areas in China showed high genetic diversity and regionally-centered differences.