CAJ | 학술논문

目的分析甲型H1N1流感病毒的基因组序列特征,阐明该毒株的遗传变异及分子特性.方法 GenBank中获取流感病毒全序列,对各段基因与已知序列进行分析比较,绘制进化树,并分析和预测甲型毒株的致病性、药物敏感性和现有疫苗的预防保护作用.结果甲型H1N1病毒的HA、PB2、PB1、PA、NP、NS基因与美国本土的猪流感病毒序列具有高度同源性,NA和M基因具有典型的欧亚株系猪流感病毒特征.该病毒具有人传人的分子基础,HA上HA1和HA2裂解位点序列为PSIQSR↓+GLFGAI,尚不具备高致病性流感病毒的特征.病毒对金刚烷胺类药物耐药,而对达菲和扎那米韦敏感.HA片段5个抗原决定区氨基酸序列与人用流感疫苗具有较大差异,推测现有疫苗对预防本次疫情基本无效.结论甲型H1N1是一种北美和欧亚两种猪流感病毒的混合体,开发针对本病毒的流感疫苗有助于进一步控制疫情蔓延.
목적 분석갑형H1N1류감병독적기인조서렬특정,천명해독주적유전변이급분자특성.방법 GenBank중획취류감병독전서렬,대각단기인여이지서렬진행분석비교,회제진화수,병분석화예측갑형독주적치병성、약물민감성화현유역묘적예방보호작용.결과 갑형H1N1병독적HA、PB2、PB1、PA、NP、NS기인여미국본토적저류감병독서렬구유고도동원성,NA화M기인구유전형적구아주계저류감병독특정.해병독구유인전인적분자기출,HA상HA1화HA2렬해위점서렬위PSIQSR↓+GLFGAI,상불구비고치병성류감병독적특정.병독대금강완알류약물내약,이대체비화찰나미위민감.HA편단5개항원결정구안기산서렬여인용류감역묘구유교대차이,추측현유역묘대예방본차역정기본무효.결론 갑형H1N1시일충북미화구아량충저류감병독적혼합체,개발침대본병독적류감역묘유조우진일보공제역정만연.
Objective To analyse the genome of influenza A (H1N1) vires so as to elucidate its molecular characteristics and evolution status. Methods DNA sequences of the influenza viruses were collected from NCBI, and compared with the genomes of referenced intluenza viruses. The phylogenetic trees were constructed by the neighbor-joining method, and the pathogenicity, drug susceptibility and vaccine protection were analyzed. Results Phyiogenetic analysis showed that the genes encoding HA, PB2, PBI, PA, NP, and NS protein were most closely related to those influenza A viruses circulating in swine populations in North America. NA and M gene belonged to Eurasia lineages swine influenza vires. The amino acid sequence of the cleavage site between HA1 and HA2 was PARSSR ↓ GLFGAI with the typical characteristics of the low pathogenic influenza virus. Influenza A(H1N1) virus can spread from person-to-person. It is sensitive to oseltamivir and zanamivir but resistant to amantadine and remantadine. The current human seasonal influenzavaccines confered little protection against influenza A/H1N1 because of the great diversity on antigenic domains between A/H1N1 virus and vaccine virus. Conclusions Influenza A(H1N1) virus is a reassortant virus of North America and Eurasia hneages swine influenza virus. It is important to develop a vaccine against the currently circulating virus strain to control the disease spread.