中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2009年
3期
155-159
,共5页
刘勇%林建东%肖雄箭%张贝蕾%林辉
劉勇%林建東%肖雄箭%張貝蕾%林輝
류용%림건동%초웅전%장패뢰%림휘
基因芯片%脓毒症%心%基因表达
基因芯片%膿毒癥%心%基因錶達
기인심편%농독증%심%기인표체
DNA microarray%sepsis%heart%gene expression
目的 应用基因芯片技术初步分析脓毒症大鼠心脏组织细胞基因表达谱的变化.方法 雄性Wistar大鼠30只被随机分为脓毒症组和对照组,每组15只.采用盲肠结扎穿孔术(CLP)制备大鼠脓毒症模型,以透射电镜下心脏组织检查鉴定模型.应用含有22 523个大鼠基因cDNA克隆的表达谱基因芯片进行检测,以Cy3和Cy5两种荧光信号强度结果 比值均>2.0或<0.5的基因为脓毒症表达差异基因,用计算机软件筛选并分析脓毒症大鼠心脏组织术后24 h的基因表达变化,并初步分析表达差异基因与脓毒症之间的关系.结果 与对照组比较,脓毒症组大鼠心脏组织共筛选出418个出现差异表达的基因,占基因芯片总点数的1.86%,其中表达上调者200个,表达下调者218个.在已知功能基因中表达上调者84个,下调者74个,与应激反应、细胞信号转导、糖皮质激素受体、免疫反应、胰岛素样生长因子、细胞物质能量代谢等方面的相关基因功能有关.结论 脓毒症大鼠心脏组织出现一系列基因表达异常,用基因芯片检测技术可快速分析.
目的 應用基因芯片技術初步分析膿毒癥大鼠心髒組織細胞基因錶達譜的變化.方法 雄性Wistar大鼠30隻被隨機分為膿毒癥組和對照組,每組15隻.採用盲腸結扎穿孔術(CLP)製備大鼠膿毒癥模型,以透射電鏡下心髒組織檢查鑒定模型.應用含有22 523箇大鼠基因cDNA剋隆的錶達譜基因芯片進行檢測,以Cy3和Cy5兩種熒光信號彊度結果 比值均>2.0或<0.5的基因為膿毒癥錶達差異基因,用計算機軟件篩選併分析膿毒癥大鼠心髒組織術後24 h的基因錶達變化,併初步分析錶達差異基因與膿毒癥之間的關繫.結果 與對照組比較,膿毒癥組大鼠心髒組織共篩選齣418箇齣現差異錶達的基因,佔基因芯片總點數的1.86%,其中錶達上調者200箇,錶達下調者218箇.在已知功能基因中錶達上調者84箇,下調者74箇,與應激反應、細胞信號轉導、糖皮質激素受體、免疫反應、胰島素樣生長因子、細胞物質能量代謝等方麵的相關基因功能有關.結論 膿毒癥大鼠心髒組織齣現一繫列基因錶達異常,用基因芯片檢測技術可快速分析.
목적 응용기인심편기술초보분석농독증대서심장조직세포기인표체보적변화.방법 웅성Wistar대서30지피수궤분위농독증조화대조조,매조15지.채용맹장결찰천공술(CLP)제비대서농독증모형,이투사전경하심장조직검사감정모형.응용함유22 523개대서기인cDNA극륭적표체보기인심편진행검측,이Cy3화Cy5량충형광신호강도결과 비치균>2.0혹<0.5적기인위농독증표체차이기인,용계산궤연건사선병분석농독증대서심장조직술후24 h적기인표체변화,병초보분석표체차이기인여농독증지간적관계.결과 여대조조비교,농독증조대서심장조직공사선출418개출현차이표체적기인,점기인심편총점수적1.86%,기중표체상조자200개,표체하조자218개.재이지공능기인중표체상조자84개,하조자74개,여응격반응、세포신호전도、당피질격소수체、면역반응、이도소양생장인자、세포물질능량대사등방면적상관기인공능유관.결론 농독증대서심장조직출현일계렬기인표체이상,용기인심편검측기술가쾌속분석.
Objective To investigate the changes in gene expression spectrum of heart tissue in septic rats by DNA microarrays. Methods Thirty male Wistar rats were randomly divided into sepsis group and control group with 15 rats in each group. Cecal ligation and puncture (CLP) was used to reproduce rat sepsis model, and the success of reproduction was confirmed by examining the heart tissue with transmission electron microscope. Gene expression spectrum was studied with oligonucleotide gene expression profile microarray that contained 22 523 rat cDNA clones to detect the changes in gene expression pattern of rat heart tissue 24 hours after CLP. Genes with fluorescent signal of Cy3/Cy5 of ratio average (RA)>2.0 or RA<0.5 were identified as differential genes, then those that high correlated to sepsis were screened by means of related computer software, and their relationship was analyzed. Results Electron microscopic examination of heart tissue demonstrated that the sepsis model was successfully reproduced. Compared to the controls, gene expression of 418 genes of heart tissue of septic rat were changed 24 hours after CLP, accounting for 1.86%, and among them 200 genes showed up-regulation, 218 genes with down-regulation. Among known functional genes, 84 genes up-regulated and 74 genes down-regulated. They were related with a range of genetic functions, such as acute stress reaction, signal transduction, immune response, energy metabolism related genes etc. Conclusion There are a series of changes in gene expression in heart tissue apparently induced by sepsis rat. DNA microarray technology provides a new tool for rapidly analyzing them.