中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
7期
14-16
,共3页
何俊瑛%张楠%邹月丽%李玉静%李美杰%张环环%谷聚贤
何俊瑛%張楠%鄒月麗%李玉靜%李美傑%張環環%穀聚賢
하준영%장남%추월려%리옥정%리미걸%장배배%곡취현
脑炎,病毒性%血脑屏障%S100蛋白质类%血管内皮生长因子A
腦炎,病毒性%血腦屏障%S100蛋白質類%血管內皮生長因子A
뇌염,병독성%혈뇌병장%S100단백질류%혈관내피생장인자A
Encephalitis,viral%Blood-brain barrier%S100 proteins%Vascular endothelial growth factor A
目的 通过测定病毒性脑炎患者脑脊液中S-100B蛋白及血管内皮生长因子(VEGF)水平,探讨其在病毒性脑炎发病过程中对脑损害的意义.方法 选择42例病毒性脑炎患者(病毒性脑炎组)和同期住院治疗的40例其他疾病患者(对照组),分别进行脑脊液常规、生化及细胞学检查,采用双抗体夹心ABC-ELISA法测定脑脊液中S-100B蛋白及VEGF水平,并进行比较.结果 病毒性脑炎组脑脊液中白细胞数为(0 ～ 584)×106/L,对照组为(0～200)×106/L.两组脑脊液中蛋白、葡萄糖水平比较差异无统计学意义(P＞0.05),氯化物水平比较差异有统计学意义[(110.10±31.22) mmol/L比(123.80±6.32) mmol/L](P=0.006).病毒性脑炎组脑脊液细胞学检查呈混合细胞学反应6例,占14.3％( 6/42).病毒性脑炎组脑脊液中S-100B蛋白水平为25.04 ～ 47.97 (28.37±6.09) ng/L,对照组为25.04～29.64( 26.03±0.90) ng/L,两组比较差异有统计学意义(t=2.462,P=0.018).病毒性脑炎组脑脊液中VEGF水平为88.84～143.77(96.24±13.38) ng/L,对照组为89.15 ～96.18(90.67±1.71) ng/L,两组比较差异有统计学意义(t=2.673,P=0.011).结论 病毒性脑炎患者脑脊液中S-100B蛋白及VEGF水平增高均可以支持病毒性脑炎的诊断,动态观察其水平变化对病毒性脑炎患者的病情监测具有重要意义.
目的 通過測定病毒性腦炎患者腦脊液中S-100B蛋白及血管內皮生長因子(VEGF)水平,探討其在病毒性腦炎髮病過程中對腦損害的意義.方法 選擇42例病毒性腦炎患者(病毒性腦炎組)和同期住院治療的40例其他疾病患者(對照組),分彆進行腦脊液常規、生化及細胞學檢查,採用雙抗體夾心ABC-ELISA法測定腦脊液中S-100B蛋白及VEGF水平,併進行比較.結果 病毒性腦炎組腦脊液中白細胞數為(0 ～ 584)×106/L,對照組為(0～200)×106/L.兩組腦脊液中蛋白、葡萄糖水平比較差異無統計學意義(P＞0.05),氯化物水平比較差異有統計學意義[(110.10±31.22) mmol/L比(123.80±6.32) mmol/L](P=0.006).病毒性腦炎組腦脊液細胞學檢查呈混閤細胞學反應6例,佔14.3％( 6/42).病毒性腦炎組腦脊液中S-100B蛋白水平為25.04 ～ 47.97 (28.37±6.09) ng/L,對照組為25.04～29.64( 26.03±0.90) ng/L,兩組比較差異有統計學意義(t=2.462,P=0.018).病毒性腦炎組腦脊液中VEGF水平為88.84～143.77(96.24±13.38) ng/L,對照組為89.15 ～96.18(90.67±1.71) ng/L,兩組比較差異有統計學意義(t=2.673,P=0.011).結論 病毒性腦炎患者腦脊液中S-100B蛋白及VEGF水平增高均可以支持病毒性腦炎的診斷,動態觀察其水平變化對病毒性腦炎患者的病情鑑測具有重要意義.
목적 통과측정병독성뇌염환자뇌척액중S-100B단백급혈관내피생장인자(VEGF)수평,탐토기재병독성뇌염발병과정중대뇌손해적의의.방법 선택42례병독성뇌염환자(병독성뇌염조)화동기주원치료적40례기타질병환자(대조조),분별진행뇌척액상규、생화급세포학검사,채용쌍항체협심ABC-ELISA법측정뇌척액중S-100B단백급VEGF수평,병진행비교.결과 병독성뇌염조뇌척액중백세포수위(0 ～ 584)×106/L,대조조위(0～200)×106/L.량조뇌척액중단백、포도당수평비교차이무통계학의의(P＞0.05),록화물수평비교차이유통계학의의[(110.10±31.22) mmol/L비(123.80±6.32) mmol/L](P=0.006).병독성뇌염조뇌척액세포학검사정혼합세포학반응6례,점14.3％( 6/42).병독성뇌염조뇌척액중S-100B단백수평위25.04 ～ 47.97 (28.37±6.09) ng/L,대조조위25.04～29.64( 26.03±0.90) ng/L,량조비교차이유통계학의의(t=2.462,P=0.018).병독성뇌염조뇌척액중VEGF수평위88.84～143.77(96.24±13.38) ng/L,대조조위89.15 ～96.18(90.67±1.71) ng/L,량조비교차이유통계학의의(t=2.673,P=0.011).결론 병독성뇌염환자뇌척액중S-100B단백급VEGF수평증고균가이지지병독성뇌염적진단,동태관찰기수평변화대병독성뇌염환자적병정감측구유중요의의.
Objective To investigate the significance of cerebrospinal fluid(CSF) S-100B protein and vascular endothelial growth factor (VEGF) levels in the pathogenesis of brain injury of viral encephalitis.Methods Forty-two patients with viral encephalitis (viral encephalitis group) and 40 patients with other disease at the corresponding time period (control group) were involved in this study.CSF (routine,biochemistry and cytology) was detected,and the levels of S-100B protein and VEGF in CSF were detected by ABC-ELISA method.Results White blood cell count was (0-584) × 106/L in viral encephalitis group,and (0-200) × 106/L in control group.The levels of protein and glucose in CSF had no significant difference between two groups (P＞ 0.05),and the level of chloride in CSF in viral encephalitis group was significantly lower than that in control group[ ( 110.10 ± 31.22 ) mmol/L vs.( 123.80 ± 6.32 ) mmol/L ] (P =0.006).In viral encephalitis group,cytological examination showed that mixed type cytological reaction was in 6 cases (14.3％,6/42).The level of S-100B protein in viral encephalitis group [25.04-47.97 (28.37 ± 6.09) ng/L] was significantly higher than that in control group[ 25.04-29.64(26.03 ± 0.90) ng/L ] (t =2.462,P =0.018).The level of VEGF in viral encephalitis group[88.84～143.77(96.24 ± 13.38) ng/L] was significantly higher than that in control group [89.15～96.18 (90.67 ± 1.71 ) ng/L] (t =2.673,P =0.011 ).Conclusions The high levels of S-100B protein and VEGF in CSF could support the viral encephalitis diagnosis.Tracking the levels of S-100B protein and VEGF in CSF dynamically have noticeable effect on checking the condition of viral encephalitis patients.
