CAJ | 학술논문

目的观察磷脂酶Cε1(PLCE1)基因过表达对结肠癌细胞生长增殖的影响.方法通过基因转染、0.7 g/L浓度的G418压力维持筛选出PLCE1过表达100倍以上的SW620结肠癌细胞株；通过噻唑蓝(MTT)生长曲线、克隆形成、裸鼠成瘤等实验观察PLCE1基因过表达对结肠癌细胞生长及增殖的影响.结果 PLCE1基因过表达导致结肠癌细胞生长速度减慢,实验组平板克隆吸光度(A)值为0.3225±0.0065,软琼脂克隆数为11.80 ±2.56,肿瘤质量(0.317±0.169)g,均较亲本组和对照组显著下降,差异有统计学意义(P＜0.05),提示转染PLCE1基因后细胞恶性程度降低.结论 PLCE1过表达抑制结肠癌细胞的生长,该基因可能是新的结直肠癌相关抑癌基因.
목적 관찰린지매Cε1(PLCE1)기인과표체대결장암세포생장증식적영향.방법 통과기인전염、0.7 g/L농도적G418압력유지사선출PLCE1과표체100배이상적SW620결장암세포주；통과새서람(MTT)생장곡선、극륭형성、라서성류등실험관찰PLCE1기인과표체대결장암세포생장급증식적영향.결과 PLCE1기인과표체도치결장암세포생장속도감만,실험조평판극륭흡광도(A)치위0.3225±0.0065,연경지극륭수위11.80 ±2.56,종류질량(0.317±0.169)g,균교친본조화대조조현저하강,차이유통계학의의(P＜0.05),제시전염PLCE1기인후세포악성정도강저.결론 PLCE1과표체억제결장암세포적생장,해기인가능시신적결직장암상관억암기인.
Objective To observe the effects of phospholipase C epsilon-1 (PLCE1) over-expression on growth and proliferation of colon cancer cells.Methods The stable SW620 colon cancer cells with 100 times of PLCE1 up-regulation were constructed by gene transfection.The effects of PLCE1 over-expression on cancer cell malignant degree were measured by methyl thiazol tetrazolium (MTT) assay,plate colony formation assay,soft agar colony formation assay and xenograft assay.Results Over-expression of PLCE1 significantly inhibited the growth and proliferation of colon cancer cells.In PLCE1 over-expression group,absorbance value of plate colony formations was 0.3225 ± 0.0065,number of soft agar colony formations was 11.80 ±2.56 and the tumor quality was (0.317 ±0.169) g.There was significant difference between experimental group and control group.The cell malignant degree after transfection was reduced.Conclusion These results suggested that PLCE1 may be involved in the development of sporadic colorectal cancer through its inhibitory effect on cell proliferation.PLCE1 exhibited a suppressive role in incidence of colorectal cancer.