CAJ | 학술논문

目的研究左旋氨氯地乎对成年大鼠神经干细胞氧/糖剥夺(OGD)损伤后细胞生长活力、增殖和凋亡情况的影响,探讨左旋氨氯地平对OGD后成年大鼠神经干细胞的保护作用.方法制备成年大鼠海马神经干细胞OGD模型,按干预的左旋氨氯地平浓度分为4组:0mol/L组(即无干预对照组)、0.5 μmol/L组、1.0 μmol/L组、5.0 mol/L组.CCK-8法检测不同浓度左旋氨氯地平对OGD后成年大鼠神经干细胞生长活力的影响:Edu荧光染色法观察不同浓度左旋氨氯地平对OGD后成年大鼠神经干细胞增殖的影响:Annexin V-FITC/PI双染流式细胞仪检测OGD后成年大鼠神经干细胞的凋亡情况. 结果 OGD6 h后成功制备成年大鼠海马神经干细胞OGD模型.与无干预对照组比较,不同浓度左旋氨氯地平干预后成年大鼠海马神经干细胞的生长活力均明显增加,差异有统计学意义(P＜0.05); 5.0 μmol/L左旋氨氯地平组OGD后神经干细胞增殖率明显增加,差异有统计学意义(P＜0.05)；1.0 μmol/L和5.0 μmol/L左旋氨氯地平组OGD后神经干细胞中凋亡细胞比例明显减少,差异有统计学意义(P＜0.05). 结论成年大鼠神经干细胞OGD模型中,左旋氨氯地平具有一定的抗OGD损伤的保护作用.
목적 연구좌선안록지호대성년대서신경간세포양/당박탈(OGD)손상후세포생장활력、증식화조망정황적영향,탐토좌선안록지평대OGD후성년대서신경간세포적보호작용.방법 제비성년대서해마신경간세포OGD모형,안간예적좌선안록지평농도분위4조:0mol/L조(즉무간예대조조)、0.5 μmol/L조、1.0 μmol/L조、5.0 mol/L조.CCK-8법검측불동농도좌선안록지평대OGD후성년대서신경간세포생장활력적영향:Edu형광염색법관찰불동농도좌선안록지평대OGD후성년대서신경간세포증식적영향:Annexin V-FITC/PI쌍염류식세포의검측OGD후성년대서신경간세포적조망정황. 결과 OGD6 h후성공제비성년대서해마신경간세포OGD모형.여무간예대조조비교,불동농도좌선안록지평간예후성년대서해마신경간세포적생장활력균명현증가,차이유통계학의의(P＜0.05); 5.0 μmol/L좌선안록지평조OGD후신경간세포증식솔명현증가,차이유통계학의의(P＜0.05)；1.0 μmol/L화5.0 μmol/L좌선안록지평조OGD후신경간세포중조망세포비례명현감소,차이유통계학의의(P＜0.05). 결론 성년대서신경간세포OGD모형중,좌선안록지평구유일정적항OGD손상적보호작용.
Objective To explore the effects of Levamlodipine on viability,proliferation and apoptosis of neural stem cells after hypoxia-ischemia injury in adult rats. Methods Hypoxic-ischemic damage to adult neural stem cells was simulated in the established oxygen/glucose deprivation (OGD)models.Four groups were designed according to Levamlodipine concentrations:0 μmol/L,0.5 μmol/L,1.0 μ mol/L and 5.0 μmol/L. Effects of Levamlodipine at 4 different concentrations on the viability,proliferation and apoptosis of hippocampal neural stem cells after OGD were tested by CCK-8 assay,Edu fluorescence staining and flow cytometry (Annexin V-FITC/PI),respectively. Results Models of hypoxia-ischemia damage to hippocampus neural stem cells were successfully established by OGD for 6hours.Compared with control group (0 μmol/L),the viability of hippocampal neural stem cells was significantly increased in the other 3 groups (0.5 μmol/L,1.0 μmol/L and 5.0 μmol/L) (P＜0.05).The proportion of proliferating cells after OGD was significantly increased at S phase in 5.0 μmol/LLevamlodipine group (P＜0.05).The proportion of apoptotic cells after OGD was significantly decreased in 1.0 μ,mol/L and 5.0μ mol/L Levamlodipine groups (P＜0.05). Conclusion Levamlodipine may protect neural stem cells from hypoxic-ischemic injury in adult rats.