CAJ | 학술논문

目的探讨内皮祖细胞(EPC)移植对睾丸扭转复位后生精功能的影响.方法获取大鼠骨髓源性EPC,采用携带增强型绿色荧光蛋白的重组腺病毒(Ad-eGFP)转染EPC.将大鼠分3组,每组各6只.假手术组:左侧睾丸未扭转;缺血再灌注损伤(IRI)组:睾丸扭转复位后经股静脉注射1 ml生理盐水;EPC组:睾丸扭转复位后注射1 ml EPC悬液(EPC数为1.0×106个).另外对3只睾丸扭转复位后大鼠进行Ad-eGFP转染后的EPC移植.术后5 d,观察移植EPC的归巢特征,并检测各组睾丸组织病理变化以及凋亡细胞/生精小管情况.结果重复感染倍数(MOI)=50时,Ad-eGFP对EPC的转染效率>73.7%.Ad-eGFP EPC移植后5 d,可在左侧睾丸组织中发现绿色荧光细胞.假手术组生精细胞结构正常,凋亡细胞/生精小管为0.09±0.02.IRI组生精细胞稀少,凋亡细胞/生精小管为2.82 ±0.81.EPC组生精上皮较IRI组显著增厚,EPC组凋亡细胞/生精小管为0.32±0.09,低于IRI组,差异有统计学意义(P＜0.01).结论 EPC移植可改善睾丸扭转导致的生精功能障碍,对生精细胞产生保护作用.
목적 탐토내피조세포(EPC)이식대고환뉴전복위후생정공능적영향.방법 획취대서골수원성EPC,채용휴대증강형록색형광단백적중조선병독(Ad-eGFP)전염EPC.장대서분3조,매조각6지.가수술조:좌측고환미뉴전;결혈재관주손상(IRI)조:고환뉴전복위후경고정맥주사1 ml생리염수;EPC조:고환뉴전복위후주사1 ml EPC현액(EPC수위1.0×106개).령외대3지고환뉴전복위후대서진행Ad-eGFP전염후적EPC이식.술후5 d,관찰이식EPC적귀소특정,병검측각조고환조직병리변화이급조망세포/생정소관정황.결과 중복감염배수(MOI)=50시,Ad-eGFP대EPC적전염효솔>73.7%.Ad-eGFP EPC이식후5 d,가재좌측고환조직중발현록색형광세포.가수술조생정세포결구정상,조망세포/생정소관위0.09±0.02.IRI조생정세포희소,조망세포/생정소관위2.82 ±0.81.EPC조생정상피교IRI조현저증후,EPC조조망세포/생정소관위0.32±0.09,저우IRI조,차이유통계학의의(P＜0.01).결론 EPC이식가개선고환뉴전도치적생정공능장애,대생정세포산생보호작용.
Objective To investigate the effects of transplanted endothelial progenitor cells (EPCs) on the spermatogenic functions in testicular detorsion. Methods Bone-marrow-derived EPCs were obtained from rats and transfected by enhanced green fluorescent protein adenovirus (Ad-eGFP). The rats were divided into 3 groups (n=6 each). In the sham group, left testis was not twisted. In the ischemia reperfusion injury (IRI) group, 1 ml saline was injected into the femoral vein of each rat after testicular detorsion. In the EPCs group, 1 ml EPCs suspension (1.0×106 EPCs) was injected into each rat after testicular detorsion. The Ad-eGFP transfected EPCs were injected into the 3 additional rats of testicular torsion-detorsion. At Day 5 post-transplantation, the characteristics of transplanted EPCs homing were detected. And the pathological changes and apoptotic cells/seminiferous tubules in left testis were examined. ResultsWhen the value of multiplication of infection (MOI) was at 50, the transfection rate of EPCs by Ad-eGFP exceeded 73.7%. At Day 5 post-treatment, the cells exhibiting green fluorescence were detected in left testis. The germ cells in rats of the sham group were normal. And the ratio of apoptotic cells to seminiferous tubules was 0.09±0.02. The germ cells in rats of the IRI group were much fewer. And the ratio of apoptotic cells to seminiferous tubules was 2.82±0.81. As compared with the IRI group, seminiferous epithelium was thicker in the EPCs group. And the ratio of apoptotic cells to seminiferous tubules was 0.32±0.09 in the EPCs group. It was much smaller than that in the IRI group. There was significant difference (P<0.01). Conclusion The transplantation of EPCs is effective for treating the spermatogenic dysfunctions caused by testicular torsion so as to greatly enhance the spermatogenic functions.