CAJ | 학술논문

目的探讨建立长期存活的人造二尖瓣植入模型的方法.方法选用健康绵羊16只,雌雄各半,预实验6只,正式实验10只.麻醉成功后建立体外循环,经降主动脉插动脉灌注管,右心耳至右心房插静脉引流管,升主动脉根部置冷灌管,经左心耳显露二尖瓣,采用间断缝合方法植入人造二尖瓣.术后常规抗生素预防感染,口服华法林抗凝.结果正式实验中成功8例并长期成活达5个月以上,术后未出现并发症,实验结束行病理检查各脏器未见异常,2例死亡.结论绵羊作为长期存活人造二尖瓣植入模型的实验动物可行可靠、存活率高、术后易于管理.
목적 탐토건립장기존활적인조이첨판식입모형적방법.방법 선용건강면양16지,자웅각반,예실험6지,정식실험10지.마취성공후건입체외순배,경강주동맥삽동맥관주관,우심이지우심방삽정맥인류관,승주동맥근부치랭관관,경좌심이현로이첨판,채용간단봉합방법식입인조이첨판.술후상규항생소예방감염,구복화법림항응.결과 정식실험중성공8례병장기성활체5개월이상,술후미출현병발증,실험결속행병리검사각장기미견이상,2례사망.결론 면양작위장기존활인조이첨판식입모형적실험동물가행가고、존활솔고、술후역우관리.
Objective To explore long-term survival of artificial mitral valve implantation model.Methods Healthy sheep (n =16,male and female,pre-test 6,the formal experiment 10 ) were used to make models.Cardiopulmonary bypass was established aftcr the success of anesthesia,artery infusion tube inserted in the descending aorta,venous drainage tube inserted through the right atrial appendage to right atrial,cold irrigation tube inserted through aortic root.Mitral valve was exposed and removed through the left atrial appendage;artificial mitral valve was implanted by interrupted suture.Antibiotic was used to prevent infection,warfarin to anticoagulant conventionally after operation.Results Eight sheep survived to full term (5 months of more) in formal experiment.No postoperative complications were observed. Conclusion This study demonstrated the feasibility and reliability of the sheep model for long-term survival of mirtal valve implantation.This sheep model provided improved rates of morbidity and mortality ease of postoperative care.