中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2009年
7期
559-564
,共6页
陈侠斌%陈光烈%刘俊鸟%杨景忠%于典科%林东昕%谭文
陳俠斌%陳光烈%劉俊鳥%楊景忠%于典科%林東昕%譚文
진협빈%진광렬%류준조%양경충%우전과%림동흔%담문
食管肿瘤%多态性,单核苷酸%蒙族
食管腫瘤%多態性,單覈苷痠%矇族
식관종류%다태성,단핵감산%몽족
Esophageal neoplasms%Polymorphism,single nucleotide%Mongolian nationality
目的 探讨中国汉族人群中食管癌遗传易感性相关的功能性单核苷酸多态(single nucleotide polymorphism,SNP)与蒙古族人群中食管癌发病风险的关系,并比较这些遗传变异在汉族人群和蒙古族人群中的等位基因频率分布差异.方法 应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-based restriction fragment length polymorphism assay,PCR-RFLP)方法,检测了188例蒙古族食管癌患者和324名蒙古族正常对照者的FAS-670G/A、FAS-1377G/A、FASL-844T/C、COX-2-1290A/G、COX-2-1195G/A、STK15 Phe31lie、MMP-2-1306C/T和MMP-2-735C/T共8个多态位点的基因型频率.采用多因素logistic回归模型计算各基因型携带者食管癌的发病风险.并将以上结果 与已报道的汉族人群中的结果 进行比较.结果 在蒙古族人群中,与携带STK1531Ile/Ile基因型者相比,携带STK15 31Phe/Phe者罹患食管癌的相对危险性增加(校正OR=2.20,95%C/值:1.12～4.31).携带MMP-2-735TT基因型者罹患食管癌的危险性明显高于携带-735CC基因型者(校正OR=4.82,95%C/值:1.59～14.60),未发现其他遗传变异与食管癌的患病风险相关.我国蒙古族和汉族人群间FASL-844 T/C[0.264(171/648)/0.736(477/648),0.323(418/1296)/0.677(878/1296)]、COX-2-1195G/A[0.431(279/648)/0.569(369/648),0.492(1250/2540)/0.508(1290/2540)]、MMP-2-1306C/T[0.869(563/648)/0.131(85/648),0.835(1298/1554)/0.165(256/1554)]和MMP-2-735C/T[0.789(511/648)/0.211(137/648),0.748(1163/1554)/0.252(391/1554)]等位基因频率分布差异有统计学意义(x2值分别为7.03、7.84、3.94、4.05,P值均<0.05).结论 STK15 Phe31Ile多态和MMP-2-735C/T可能是我国蒙古族人群食管癌的遗传易感因素.我国蒙古族和汉族人群的遗传差异导致不同SNP与食管癌发病风险关联情况存在差别.
目的 探討中國漢族人群中食管癌遺傳易感性相關的功能性單覈苷痠多態(single nucleotide polymorphism,SNP)與矇古族人群中食管癌髮病風險的關繫,併比較這些遺傳變異在漢族人群和矇古族人群中的等位基因頻率分佈差異.方法 應用聚閤酶鏈反應-限製性片段長度多態性(polymerase chain reaction-based restriction fragment length polymorphism assay,PCR-RFLP)方法,檢測瞭188例矇古族食管癌患者和324名矇古族正常對照者的FAS-670G/A、FAS-1377G/A、FASL-844T/C、COX-2-1290A/G、COX-2-1195G/A、STK15 Phe31lie、MMP-2-1306C/T和MMP-2-735C/T共8箇多態位點的基因型頻率.採用多因素logistic迴歸模型計算各基因型攜帶者食管癌的髮病風險.併將以上結果 與已報道的漢族人群中的結果 進行比較.結果 在矇古族人群中,與攜帶STK1531Ile/Ile基因型者相比,攜帶STK15 31Phe/Phe者罹患食管癌的相對危險性增加(校正OR=2.20,95%C/值:1.12～4.31).攜帶MMP-2-735TT基因型者罹患食管癌的危險性明顯高于攜帶-735CC基因型者(校正OR=4.82,95%C/值:1.59～14.60),未髮現其他遺傳變異與食管癌的患病風險相關.我國矇古族和漢族人群間FASL-844 T/C[0.264(171/648)/0.736(477/648),0.323(418/1296)/0.677(878/1296)]、COX-2-1195G/A[0.431(279/648)/0.569(369/648),0.492(1250/2540)/0.508(1290/2540)]、MMP-2-1306C/T[0.869(563/648)/0.131(85/648),0.835(1298/1554)/0.165(256/1554)]和MMP-2-735C/T[0.789(511/648)/0.211(137/648),0.748(1163/1554)/0.252(391/1554)]等位基因頻率分佈差異有統計學意義(x2值分彆為7.03、7.84、3.94、4.05,P值均<0.05).結論 STK15 Phe31Ile多態和MMP-2-735C/T可能是我國矇古族人群食管癌的遺傳易感因素.我國矇古族和漢族人群的遺傳差異導緻不同SNP與食管癌髮病風險關聯情況存在差彆.
목적 탐토중국한족인군중식관암유전역감성상관적공능성단핵감산다태(single nucleotide polymorphism,SNP)여몽고족인군중식관암발병풍험적관계,병비교저사유전변이재한족인군화몽고족인군중적등위기인빈솔분포차이.방법 응용취합매련반응-한제성편단장도다태성(polymerase chain reaction-based restriction fragment length polymorphism assay,PCR-RFLP)방법,검측료188례몽고족식관암환자화324명몽고족정상대조자적FAS-670G/A、FAS-1377G/A、FASL-844T/C、COX-2-1290A/G、COX-2-1195G/A、STK15 Phe31lie、MMP-2-1306C/T화MMP-2-735C/T공8개다태위점적기인형빈솔.채용다인소logistic회귀모형계산각기인형휴대자식관암적발병풍험.병장이상결과 여이보도적한족인군중적결과 진행비교.결과 재몽고족인군중,여휴대STK1531Ile/Ile기인형자상비,휴대STK15 31Phe/Phe자리환식관암적상대위험성증가(교정OR=2.20,95%C/치:1.12～4.31).휴대MMP-2-735TT기인형자리환식관암적위험성명현고우휴대-735CC기인형자(교정OR=4.82,95%C/치:1.59～14.60),미발현기타유전변이여식관암적환병풍험상관.아국몽고족화한족인군간FASL-844 T/C[0.264(171/648)/0.736(477/648),0.323(418/1296)/0.677(878/1296)]、COX-2-1195G/A[0.431(279/648)/0.569(369/648),0.492(1250/2540)/0.508(1290/2540)]、MMP-2-1306C/T[0.869(563/648)/0.131(85/648),0.835(1298/1554)/0.165(256/1554)]화MMP-2-735C/T[0.789(511/648)/0.211(137/648),0.748(1163/1554)/0.252(391/1554)]등위기인빈솔분포차이유통계학의의(x2치분별위7.03、7.84、3.94、4.05,P치균<0.05).결론 STK15 Phe31Ile다태화MMP-2-735C/T가능시아국몽고족인군식관암적유전역감인소.아국몽고족화한족인군적유전차이도치불동SNP여식관암발병풍험관련정황존재차별.
Objective To investigate the frequencies of alleles and the association with risk of esophageal cancer in a Mongolian population, and to compare the allele frequencies of these polymorphisms between the two populations and the susceptibility to esophageal cancer. Methods A case-control study was conducted,and 8 single nucleotide polymorphisms (SNP) ,including FAS -670G/A, FAS -1377G/A, FASL -844T/C, COX-2 -1290A/G, COX-2 -1195G/A, STK15 Phe31Ile, MMP-2 -1306C/T and MMP -2 -735C/T,were detected by polymerase chain reaction-based restriction fragment length polymorphism assay (PCR-RFLP) in 188 esophageal cancer cases and 324 normal controls of Mongolian. The odds ratios (Ors) and 95% confidence intervals (Cis) were calculated by unconditional logistic regression. The results were then compared with the reported data of the Han ethnic Chinese population. Results In Mongolian, as compared with the STKI5 31Ile/Ile genotype,tbe STK15 31Phe/Phe genotype carriers had an increased risk of esophageal cancer (adjusted OR=2. 20,95% CI: 1.12 -4. 31) ,and the subjects with MMP-2 -735Trgenotype had an increased risk of esophageal cancer as compared with those with the MMP-2 -735CC genotype (adjusted OR=4. 82,95% CI: 1.59-14. 60). However,the rest of SNPs were not associated with the susceptibility to esophageal cancer. The allele frequencies of FASL -844 T/C [0. 264 (171/648)/0. 736 (477/648) ,0. 323 (418/1296)/0. 677 (878/1296)], COX-2 -1195G/A [0. 431 (279/648)/0. 569 (369/ 648), 0. 492 (1250/2540)/0. 508 (1290/2540)], MMP-2 -1306C/T [0. 869 (563/648)/0. 131 (85/648) ,0. 835 (1298/1554)/0. 165 (256/1554)]and MMP-2 -735C/T [0. 789 (511/648)/0. 211 (137/648) ,0. 748 (1163/1554)/0. 252 (391/1554)]were significantly different between the ethnic populations (X2=7.03,7. 84,3.94,4. 05,respectively,P<0. 05). Conclusion These findings suggested that STK15 Phe31Ile and MMP-2 -735C/T polymorphisms might be the genetic susceptibility factors for esophageal cancer in Mongolian and there should be some differences of genetic susceptibility to esophageal cancer in between Han ethnic Chinese and Mongolian population.
