CAJ | 학술논문

本文采用集成有射频功能的片上系统(nRF24E1),研制了一种用于自由活动小动物的微型多模式遥控刺激器.刺激参数的设置及结果分析均由10 m外的个人计算机进行.通过离体实验,即刺激蛙坐骨神经干时产生动作电位并可观察到伪迹;及在体大鼠的训练操作性条件反射的行为学实验,即在Y臂迷宫中训练大鼠听到不同声音完成左右运动,如果完成正确给予前脑内侧束以电刺激,我们观察到5 d内大鼠的正确率增加3倍,达到93.5%.上述实验都验证了该刺激器的有效性与稳定性.本系统具有体积小(18 mm×28 mm双层线路板)、重量轻(不含电池5 g)、简单、实用、可靠等特点,能有效地用于自由活动小动物的实验研究.为基于电刺激的小动物行为训练(动物机器人)及相关神经生理学实验研究,提供了新的实验条件和实验手段.
본문채용집성유사빈공능적편상계통(nRF24E1),연제료일충용우자유활동소동물적미형다모식요공자격기.자격삼수적설치급결과분석균유10 m외적개인계산궤진행.통과리체실험,즉자격와좌골신경간시산생동작전위병가관찰도위적;급재체대서적훈련조작성조건반사적행위학실험,즉재Y비미궁중훈련대서은도불동성음완성좌우운동,여과완성정학급여전뇌내측속이전자격,아문관찰도5 d내대서적정학솔증가3배,체도93.5%.상술실험도험증료해자격기적유효성여은정성.본계통구유체적소(18 mm×28 mm쌍층선로판)、중량경(불함전지5 g)、간단、실용、가고등특점,능유효지용우자유활동소동물적실험연구.위기우전자격적소동물행위훈련(동물궤기인)급상관신경생이학실험연구,제공료신적실험조건화실험수단.
This paper presents a remote controlled multimode micro-stimulator based on the chip nRF24E1, which consists mainly of a micro-control unit (MCU) and a radio frequency (RF) transceiver. This micro-stimulator is very compact (18 mm×28 mm two layer printed circuit board) and light (5 g without battery), and can be carried on the back of a small animal to generate electrical stimuli according to the commands sent from a PC 10 meters away. The performance and effectiveness of the micro-stimulator were validated by in vitro experiments on the sciatic nerve (SN) of the frog, where action potentials (APs) as well as artifacts were observed when the SN was stimulated by the micro-stimulator. It was also shown by in vivo behavioral experiments on operant conditioned reflexes in rats medial forebrain bundle (MFB) in a maze. The correct response for the rats to obey the instructions increased by three times and but also provide a new platform for behavioral and neurophysiological experiments.