CAJ | 학술논문

采用两步外植体法,即以百合鳞片叶为初始外植体,以从初始外植体上长出的芽为次级外植体,成功建立了麝香百合的高频离体再生系统.对不同的BA浓度及次级外植体的不同部位对再生效果的影响,以及组培苗移栽前低温处理的影响进行了研究.结果表明:不同部位的次级外植体中,以短缩茎切片出芽快、整齐、芽数多且粗壮;以MS附加1.0mgL1BA和0.1 mgL-1NAA的培养基最适于麝香百合的分化.一个中等大小已脱春化的鳞茎通过两步外植体法能扩繁出54000株左右的新植株,从鳞片叶开始至开花仅需8个月,而且4℃低温处理对开花期的影响不大.
채용량보외식체법,즉이백합린편협위초시외식체,이종초시외식체상장출적아위차급외식체,성공건립료사향백합적고빈리체재생계통.대불동적BA농도급차급외식체적불동부위대재생효과적영향,이급조배묘이재전저온처리적영향진행료연구.결과표명:불동부위적차급외식체중,이단축경절편출아쾌、정제、아수다차조장;이MS부가1.0mgL1BA화0.1 mgL-1NAA적배양기최괄우사향백합적분화.일개중등대소이탈춘화적린경통과량보외식체법능확번출54000주좌우적신식주,종린편협개시지개화부수8개월,이차4℃저온처리대개화기적영향불대.
Bulb scale segments of Lilium longiflorum were used as original explants and shoot sections excised from shoot clusters arosed from the first explants as the secondary explants. The effects of different concentrations of BA (benzyladenine) and different parts of plant segments on shoot formation were studied. After roots formed,plantlets were treated at 4℃ for 0, 7 and 30 days. The results indicated that MS medium supplemented with 1.0 mg L-1 BA and 0.1 mg L-1 NAA was optimum for shoot formation and proliferation by using short basal stem segment as the second explant. Moreover, cold treatment had little effect on flowering stage. About 54 000 new plantlets could be obtained from one bulb. It took only 8 months from bulb scale to flowering.