CAJ | 학술논문

背景与目的:BCR/ABL诱导多聚胞嘧啶结合蛋白E2[poly(rC)-binding protein E2,hnRNP E2]的异常表达在慢性粒细胞白血病(chronic myeloid leukemia,CML)急变中起着重要作用.本研究探讨hnRNP E2诱骗RNA(decoy RNA)对人白血病细胞K562增殖的影响,并初步探讨其可能的分子机制.方法:构建能转录出hnRNP E2诱骗RNA的质粒载体,将其经阳离子脂质体介导转染K562细胞,用G418筛选出稳定表达的细胞,台盼蓝法检测细胞的增殖能力,流式细胞仪分析细胞周期,并用RT-PCR和Western blot方法检测下游CCAAT增强子结合蛋白α(CCAAT/enhancer-binding protein α,C/EBP α)和c-myc的表达.结果:稳定表达诱骗RNA的K562细胞增殖受抑,增殖抑制率为(62.73±12.92)%;细胞周期阻滞于S期[稳定表达诱骗RNA细胞组(55.59±4.67)%,对照组(44.70±4.21)%,P＜0.05];C/EBPα mRNA无改变,但在蛋白水平42 ku-C/EBP α表达升高了(49.72±5.58)%;c-myc mRNA下降了(58.27±7.23)%,蛋白水平降低了(57.26±6.52)%.结论:hnRNP E2诱骗RNA能抑制K562细胞的增殖,其机制可能与诱骗RNA阻断hnRNP E2和C/EBPα mRNA的结合后,引起42 ku-C/EBPα表达升高有关.
배경여목적:BCR/ABL유도다취포밀정결합단백E2[poly(rC)-binding protein E2,hnRNP E2]적이상표체재만성립세포백혈병(chronic myeloid leukemia,CML)급변중기착중요작용.본연구탐토hnRNP E2유편RNA(decoy RNA)대인백혈병세포K562증식적영향,병초보탐토기가능적분자궤제.방법:구건능전록출hnRNP E2유편RNA적질립재체,장기경양리자지질체개도전염K562세포,용G418사선출은정표체적세포,태반람법검측세포적증식능력,류식세포의분석세포주기,병용RT-PCR화Western blot방법검측하유CCAAT증강자결합단백α(CCAAT/enhancer-binding protein α,C/EBP α)화c-myc적표체.결과:은정표체유편RNA적K562세포증식수억,증식억제솔위(62.73±12.92)%;세포주기조체우S기[은정표체유편RNA세포조(55.59±4.67)%,대조조(44.70±4.21)%,P＜0.05];C/EBPα mRNA무개변,단재단백수평42 ku-C/EBP α표체승고료(49.72±5.58)%;c-myc mRNA하강료(58.27±7.23)%,단백수평강저료(57.26±6.52)%.결론:hnRNP E2유편RNA능억제K562세포적증식,기궤제가능여유편RNA조단hnRNP E2화C/EBPα mRNA적결합후,인기42 ku-C/EBPα표체승고유관.