CAJ | 학술논문

AIM: To examine human β-defensin-3 (hBD-3)expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H pylori infection for better understanding the innate immune response to H pylori.METHODS: We used reverse transcription-polymerase chain reactions and immunohistochemistry to examine hBD-3 expression in inflamed gastric mucosal tissues or MKN45 gastric cancer cells with or without H pylori.Effects of hBD-3 against H pylori were also evaluated.RESULTS: The mean mRNA expression of hBD-3 in H pylori-positive specimens was significantly higher than that in H pylori-negative specimens (P = 0.0002,Mann-Whitney). In addition, unlike uninfected samples,8 of 15 (53.33%) infected mucosal samples expressed hBD-3 protein. H pylori dose-dependently induced mRNA expression of hBD-3 in MKN45 cells, an effect inhibited by adding anti-toil-like receptor (TLR)-4 antibody. HBD-3 protein completely inhibited H pylori growth.CONCLUSION: Our results suggest that like hBD-2,hBD-3 may be involved in the pathophysiology of H pylori-induced gastritis.