CAJ | 학술논문

光合气体交换参数是用来表示植物光合能力的常用指标.利用来自大豆品种科丰1号和南农1138-2的重组自交系群体NJRIKY(184个家系)及其分子遗传图谱,通过两年盆栽试验定位与光合速率、气孔导度、胞间CO_2浓度和蒸腾速率有关的QTL.结果表明.4个参数的遗传力中等偏低,在0.48～0.60之间;两两间存在极显著正相关关系,相关系数在0.192～0.686之间;两年共检测到15个QTL,分别位于C1、C2、D2、E、H、I和O连锁群上,LOD值在2.25～6.31之间,贡献率为4.80%～12.30%;有6个QTL在不同环境下稳定表达,它们分别是控制光合速率的qPnCI.1、控制气孔导度的qSCD2.1和qSCI.1、控制胞间CO_2浓度的qCiI.1和qCiO.1,以及控制蒸腾速率的qTrO.1;检测到4个同时控制两个或两个以上参数的标记区间,它们分别是C1连锁群上控制光合速率和气孔导度的sat_311～set_191区间,E连锁群上控制光合速率、气孔导度和胞间CO_2浓度的sat_172～satt268区间,I连锁群上控制气孔导度和胞间CO_2浓度的satt726～satt330区间,以及D2连锁群上控制气孔导度和胞间CO_2浓度的sat_296～sat_277区间.
광합기체교환삼수시용래표시식물광합능력적상용지표.이용래자대두품충과봉1호화남농1138-2적중조자교계군체NJRIKY(184개가계)급기분자유전도보,통과량년분재시험정위여광합속솔、기공도도、포간CO_2농도화증등속솔유관적QTL.결과표명.4개삼수적유전력중등편저,재0.48～0.60지간;량량간존재겁현저정상관관계,상관계수재0.192～0.686지간;량년공검측도15개QTL,분별위우C1、C2、D2、E、H、I화O련쇄군상,LOD치재2.25～6.31지간,공헌솔위4.80%～12.30%;유6개QTL재불동배경하은정표체,타문분별시공제광합속솔적qPnCI.1、공제기공도도적qSCD2.1화qSCI.1、공제포간CO_2농도적qCiI.1화qCiO.1,이급공제증등속솔적qTrO.1;검측도4개동시공제량개혹량개이상삼수적표기구간,타문분별시C1련쇄군상공제광합속솔화기공도도적sat_311～set_191구간,E련쇄군상공제광합속솔、기공도도화포간CO_2농도적sat_172～satt268구간,I련쇄군상공제기공도도화포간CO_2농도적satt726～satt330구간,이급D2련쇄군상공제기공도도화포간CO_2농도적sat_296～sat_277구간.
Photosynthesis plays an important role in yield forming in crops. Photosynthetic gas-exchange parameters have been widely used to reflect the photosynthetic capacity of plant. The present study aimed to identify QTLs associated with gas-exchange parameters in soybean. Pot experiments were carded out in two successive years to evaluate the photosynthetic rate, stomatal conductance, intercellar CO_2 concentration and transpiration rate at R6 growth stage in 184 recombinant inbred lines (RILs) derived from a cross between Kefeng 1 and Nannong 1138-2. The RILs showed transgressive segregation in these parameters with low to middle broad heritability, which ranged from 0.48 to 0.60. A total of 15 QTLs located on seven linkage groups (LGs) were identified, and explained phenotypic variation ranging from 4.80% to 12.30% with the LOD score from 2.25 to 6.31. Three QTLs for photosynthetic rate were located on LGs CI, E, and O; four QTLs for stomatal conductance located on LGs C1, D2, E, and I; five QTLs for intercellular CO_2 concentration located on LGs C1, D2, E, I, and O; three QTLs for transpiration rate located on LGs C2, H, and O. Six QTLs were detected in both environments, including qPnCI.1 (between markers sat_311 and sct_191) for photosynthetic rate, qSCD2.1 (flanked by sat_296 and sat_277) and qSCI.1 (flanked by satt726 and satt330) for stomatal conductance, qCiI.1 (flanked by satt726 and satt330) and qCiO.1 (flanked by satt94 and sat_291) for intercellular CO_2 concentration, qTrO.1 (flanked by BE801128 and satt345) for transpiration rate. Some QTLs for different traits were found to locate in same regions on LGs. Four genomic regions were detected to be associated with several parameters simultaneously, such as, marker interval between sat_311 and sct_ 191 on LG C1 for photosynthetic rate and stomatai conductance with positive alleles from Nannong 1138-2; marker interval between sat_172 and satt268 on LG E for photosynthetic rate, intercellular CO_2 concentration, and stomatal conductance with positive alleles from Kefeng 1; marker intervals between sat_296 and sat_277 on LG D2 and between satt726 and satt330 on LG I for stomatal conductance and interncellular CO_2 concentration with positive alleles from Nanong 1138-2 and Kefeng 1, respectively. No common QTLs that expressed stably across genotypes were found in this study. This implied that the genetic mechanism of photosynthetic gas-exchange parameters is complex in soybean, and further studies should be performed with more soybean mapping populations in future.