CAJ | 학술논문

目的:研究耐力训练对大鼠骨骼肌脂肪酸转位酶/CD36(FAT/CD36)表达的影响,并分析其与胰岛素敏感性指数(ISI)的相关关系.方法:将23只SD大鼠随机分成耐力训练组(T)(n=13)和安静对照组(C)(n=10).T组大鼠每周进行6天、共7周的中等强度跑台耐力训练.'7周后取肾周和睾丸周围脂肪组织称重,计算脂肪垫百分比;空腹取血测定总胆固醇(TC)、甘油三酯(TG)、非酯化脂肪酸(NEFA)、胰岛素(FINS)含量;取股直肌测定匀浆液FAT/CD36蛋白表达,并与ISI进行相关分析.结果:7周耐力训练后,T组大鼠体重和脂肪垫百分比均低于C组(P<0.01,P<0.01);T组大鼠血清TC、NEFA、FINS低于C组(P<0.01,P<O.05,P<O.05);T组大鼠股直肌匀浆液FAT/CD36蛋白含量、胰岛素敏感性指数(ISI)显著高于C组(P<0.01,P<0.05),且二者呈高度正相关(r=0.823,P<0.01).结论:7周耐力训练可提高大鼠胰岛素敏感性,上调FAT/CD36的表达,以适应其介导的耐力训练脂肪分解代谢增强后骨骼肌脂肪酸转运的需求.
목적:연구내력훈련대대서골격기지방산전위매/CD36(FAT/CD36)표체적영향,병분석기여이도소민감성지수(ISI)적상관관계.방법:장23지SD대서수궤분성내력훈련조(T)(n=13)화안정대조조(C)(n=10).T조대서매주진행6천、공7주적중등강도포태내력훈련.'7주후취신주화고환주위지방조직칭중,계산지방점백분비;공복취혈측정총담고순(TC)、감유삼지(TG)、비지화지방산(NEFA)、이도소(FINS)함량;취고직기측정균장액FAT/CD36단백표체,병여ISI진행상관분석.결과:7주내력훈련후,T조대서체중화지방점백분비균저우C조(P<0.01,P<0.01);T조대서혈청TC、NEFA、FINS저우C조(P<0.01,P<O.05,P<O.05);T조대서고직기균장액FAT/CD36단백함량、이도소민감성지수(ISI)현저고우C조(P<0.01,P<0.05),차이자정고도정상관(r=0.823,P<0.01).결론:7주내력훈련가제고대서이도소민감성,상조FAT/CD36적표체,이괄응기개도적내력훈련지방분해대사증강후골격기지방산전운적수구.
Objective To determine effect of endurance training on the expression of fatty acid translocase FAT/ CD36 in skeletal muscle of rats and the correlation between the expression and insulin sensitivity index (ISI). Methods Male Sprague-Dawley rats were randomly assigned to two groups:training group(T,n=13) and control group (C, n=10). Rats in group T were trained on treadmill six days a week for 7 weeks. Fat pads around testes and kidneys were weighted for calculating the percentage of fat pads vs body weight. Serum total cholesterol (TC), triglyceride (TG),nonesterified fatty acids(NEFA) and fasting insulin(FINS) were measured. Western blot was used to detect FAT/ CD36 protein expression in quadriceps homogenate. The relation of FAT/CD36 expression to the ISI was analyzed. Results Markedly lower body weight,percentage of fat pads, TC,NEFA, and FINS were seen in group T after 7-week endurance training comparing to that in group C(P<0.01 ,P<0.01 ,P<0.01 ,P<0.05,and P<0.05,respectively). The FAT/CD36 protein expression and ISI were significantly higher in group T than in group C(P<0.01and P<0.05). A significant correlation between FAT/CD36 and ISI were found in group T(r=0.823,P<0.01 ),nut not in group C. Conclusion Seven-week endurance training improved insulin sensitivity and up-regulated the expression of FAT/CD36 for adapting the requirement of fatty acid transportation in skeletal muscle induced by enhanced lipid catabolism after endurance exercise.