CAJ | 학술논문

[目的]对不同小麦品种的遗传差异进行客观的评估.[方法] 提取21种小麦幼苗总DNA,利用基于小麦反转录转座子Wis2-1A的分子标记技术,作IRAP分析,对其遗传多态性进行评价.[结果] 电泳结果进行聚类分析,有19个品种聚成不同遗传距离的类群,绝大多数小麦品种得到了充分的区分,对不同品种的小麦遗传多样性进行了客观评价.[结论] 基于小麦反转录转座子Wis2-1A的IRAP分析,可作为小麦育种判断遗传距离的依据.
[목적]대불동소맥품충적유전차이진행객관적평고.[방법] 제취21충소맥유묘총DNA,이용기우소맥반전록전좌자Wis2-1A적분자표기기술,작IRAP분석,대기유전다태성진행평개.[결과] 전영결과진행취류분석,유19개품충취성불동유전거리적류군,절대다수소맥품충득도료충분적구분,대불동품충적소맥유전다양성진행료객관평개.[결론] 기우소맥반전록전좌자Wis2-1A적IRAP분석,가작위소맥육충판단유전거리적의거.
[Objective] To analyze genetic polymorphism of different species of wheat. [Method] The DNA of young seedlings from 21 species of wheat was isolated,and their genetic polymorphism was analyzed by inter-retrotransposon amplified polymorphism (IRAP) using a molecule marker technology based on wheat retrotransposon Wis2-1 A. [Result] As shown by clustering map of the electrophoresis results,19 species of wheat assembled as cluster with different genetic distance. Most of the wheat species were distinguished. The genetic polymorphism among different species of wheat could be evaluated by this method objectively. [Conclusion] The analysis of IRAP based on wheat retrotransposon Wis2-1A could give a basis for breeding of wheat.