Foxp3,Galectin-9,PD-L1在小鼠DC2.4细胞的表达
Foxp3,Galectin-9,PD-L1재소서DC2.4세포적표체
Expression of Foxp3,Galectin-9 and PD-L1 in mouse DC2.4 cell line
  • 万方数据
    军医进修学院学报 군의진수학원학보
    ACADEMIC JOURNAL OF PLA POSTGRADUATE MEDICAL SCHOOL
    2011年 1期 75-77 ,共3页

    宫玉波%黄一飞%韩根成%李育蓉%李新颖%王大江%杜改萍%余继锋%宋静

    궁옥파%황일비%한근성%리육용%리신영%왕대강%두개평%여계봉%송정

    DC2.4%Foxp3%半乳糖凝集素-9%程序化死亡因子配体1 DC2.4%Foxp3%半乳糖凝集素-9%程序化死亡因子配體1
    DC2.4%Foxp3%반유당응집소-9%정서화사망인자배체1
    目的 观察CD80,CD86,Foxp3,Galectin-9,PD-L1在小鼠DC细胞的表达,了解小鼠DC2.4细胞系的免疫学特性.方法 用含10%胎牛血清的RPMI-1640培养液培养小鼠DC2.4细胞,光学显微镜观察细胞形态;流式细胞术检测CD80,CD86,Foxp3,Galectin-9,PD-L1在DC2.4细胞表达.结果 光学显微镜示小鼠DC2.4细胞贴壁生长,呈树突、梭形及不规则形;流式细胞术检测DC2.4细胞CD80表达为91.04%±4.90%,CD86表达为98.54%±1.96%,Foxp3无或微表达,Galectin-9表达为14.33%±2.94%,PD-LI表达为98.80%±0.63%.结论 小鼠DC2.4细胞是一个高表达CD80,CD86及PD-L1,低表达Galectin-9,无或微表达Foxp3的细胞系.
    목적 관찰CD80,CD86,Foxp3,Galectin-9,PD-L1재소서DC세포적표체,료해소서DC2.4세포계적면역학특성.방법 용함10%태우혈청적RPMI-1640배양액배양소서DC2.4세포,광학현미경관찰세포형태;류식세포술검측CD80,CD86,Foxp3,Galectin-9,PD-L1재DC2.4세포표체.결과 광학현미경시소서DC2.4세포첩벽생장,정수돌、사형급불규칙형;류식세포술검측DC2.4세포CD80표체위91.04%±4.90%,CD86표체위98.54%±1.96%,Foxp3무혹미표체,Galectin-9표체위14.33%±2.94%,PD-LI표체위98.80%±0.63%.결론 소서DC2.4세포시일개고표체CD80,CD86급PD-L1,저표체Galectin-9,무혹미표체Foxp3적세포계.
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