陕西师范大学学报：自然科学版
陝西師範大學學報：自然科學版
협서사범대학학보：자연과학판
Journal of Shaanxi Normal University： Nat Sci Ed
2012年
1期
63-66
,共4页
绞股蓝%多糖%脱蛋白%相对分子质量%高效液相色谱
絞股藍%多糖%脫蛋白%相對分子質量%高效液相色譜
교고람%다당%탈단백%상대분자질량%고효액상색보
Gynostemma pentaphyllum%polysaccharide%deproteinization%the relative molecular mass%HPLC（High Performance Liquid Chromatography）
采用Sevag法、木瓜蛋白酶-Sevag法、TCA-Sevag法对绞股蓝粗多糖脱蛋白工艺进行研究.通过DEAE—52纤维素柱层析和葡聚糖凝胶G—200柱层析对粗多糖进行分离纯化,采用高效液相色谱分析法对精制多糖GPM—21的相对分子质量进行测定分析.结果表明：木瓜蛋白酶-Sevag法效果最佳,蛋白脱除率高且多糖损失量小,累计蛋白脱除率可达63.08%,多糖保留率为77.34%.液相渗透色谱法测定GPM—21的相对分子质量为200 576.
採用Sevag法、木瓜蛋白酶-Sevag法、TCA-Sevag法對絞股藍粗多糖脫蛋白工藝進行研究.通過DEAE—52纖維素柱層析和葡聚糖凝膠G—200柱層析對粗多糖進行分離純化,採用高效液相色譜分析法對精製多糖GPM—21的相對分子質量進行測定分析.結果錶明：木瓜蛋白酶-Sevag法效果最佳,蛋白脫除率高且多糖損失量小,纍計蛋白脫除率可達63.08%,多糖保留率為77.34%.液相滲透色譜法測定GPM—21的相對分子質量為200 576.
채용Sevag법、목과단백매-Sevag법、TCA-Sevag법대교고람조다당탈단백공예진행연구.통과DEAE—52섬유소주층석화포취당응효G—200주층석대조다당진행분리순화,채용고효액상색보분석법대정제다당GPM—21적상대분자질량진행측정분석.결과표명：목과단백매-Sevag법효과최가,단백탈제솔고차다당손실량소,루계단백탈제솔가체63.08%,다당보류솔위77.34%.액상삼투색보법측정GPM—21적상대분자질량위200 576.
The optimum technologies of deproteinization using Sevag method,Papain-Sevag method and TCA-Sevag method were studied.In addition,crude polysaccharides were isolated and purified by DEAE—52 cellulose column chromatography,and Sephadex G—200 column chromatography,and relative molecular mass of refined polysaccharides GPM—21 were measured by HPLC.The results indicated that papain-Sevag method was the best method for the deproteinization in among of methods.The removal rate of total protein could reach up to 63.08%,and the retention rate of polysaccharide was 77.34%.The relative molecular mass of GPM—21 was 200 576.
