CAJ | 학술논문

目的探讨甲状腺肿瘤中3号染色体短臂(3p)杂合性缺失(LOH)状态及其临床意义.方法收集74例甲状腺肿瘤标本,包括20例甲状腺腺瘤(FA)、24例滤泡性甲状腺癌(FTC)和30例乳头状甲状腺癌(Prc).通过PCR扩增和银染分析其3p上11个微卫星位点的杂合性缺失状态.结果 FFC的LOH频率达到71%(17/24),PTC中30%(9/30),FA中10%(2/20).FFC的3p LOH频率显著高于FA和PTC(P<0.01).FTC中存在两个最小共同缺失区,分别位于3p26-pter和3p14.2-3p22.PTC上存在一个最小共同缺失区,位于3p 25.2-26.1.结论 FTC的3p LOH频率显著高于FA和PTC.3p的3个最小缺失区上可能存在着与FTC和PTC发生发展相关的肿瘤抑制基因.
목적 탐토갑상선종류중3호염색체단비(3p)잡합성결실(LOH)상태급기림상의의.방법 수집74례갑상선종류표본,포괄20례갑상선선류(FA)、24례려포성갑상선암(FTC)화30례유두상갑상선암(Prc).통과PCR확증화은염분석기3p상11개미위성위점적잡합성결실상태.결과 FFC적LOH빈솔체도71%(17/24),PTC중30%(9/30),FA중10%(2/20).FFC적3p LOH빈솔현저고우FA화PTC(P<0.01).FTC중존재량개최소공동결실구,분별위우3p26-pter화3p14.2-3p22.PTC상존재일개최소공동결실구,위우3p 25.2-26.1.결론 FTC적3p LOH빈솔현저고우FA화PTC.3p적3개최소결실구상가능존재착여FTC화PTC발생발전상관적종류억제기인.
Objective To study the loss of heterozygosity(LOH)on chromosome 3p in thyroid tumors. Methods LOH at 11 microsatellite loci Wag analyzed in 74 cases of thyroid tumors(including 20 follicular adenomas, 24 follicular thyroid carcinomas and 30 papillary thyroid carcinomas)by polymerase chain reaction and silver stain. Results LOH on chromosome 3p Wag detected in 71% of follicular thyroid carcinoma(17/24), 30%of the papillary thyroid carcinoma(9/30) and 10%of the follicular adenoma (2/20)case. Two minimal common deleted regions(CDR)(3p26-pter and 3p14. 2-3p22)involving significant sites of LOH has identified in follicular thyroid carcinoma. There Was also one CDR(3p25. 2-26. 1)in papilhry thyroid careinoma. Conclusions LOH is more frequently identified in follicular thyroid careinoma than in papillary thyroid carcinoma and follicular adenoma. The 3 CDR on chromosome 3p may harbor tumor suppressor genes involved in the pathogenesis of follicular thyroid carcinoma and papillary thyroid carcinoma.