CAJ | 학술논문

目的探讨miR-21和miR-125在结直肠癌中的表达与临床病理特征之间的关系.方法选取2006年7月至2007年12月经手术切除的原发性结直肠癌标本100例,采用实时荧光定量PCR分析miR-21和miR-125的表达情况.结果相对于癌旁组织,100例结直肠癌组织中miR-21表达上调2.3倍(P=0.025),其表达与TNM分期(P=0.028)以及浸润深度有关(P=0.023);而miR-125在癌组织中的表达则下调3.3倍(P=0.005),其表达与TNM分期、浸润深度和远处转移无明显关联(P>0.05).结论 miR-21可能参与了结直肠癌的发生和发展;而miR-125可能未参与.
목적 탐토miR-21화miR-125재결직장암중적표체여림상병리특정지간적관계.방법 선취2006년7월지2007년12월경수술절제적원발성결직장암표본100례,채용실시형광정량PCR분석miR-21화miR-125적표체정황.결과 상대우암방조직,100례결직장암조직중miR-21표체상조2.3배(P=0.025),기표체여TNM분기(P=0.028)이급침윤심도유관(P=0.023);이miR-125재암조직중적표체칙하조3.3배(P=0.005),기표체여TNM분기、침윤심도화원처전이무명현관련(P>0.05).결론 miR-21가능삼여료결직장암적발생화발전;이miR-125가능미삼여.
Objective To investigate the expression of microRNA(miR)-21 and miR-125 in colorectal cancer (CRC) and its relationship with clinicopathological features. Methods Quantitative real-time PCR was applied to examine the expression of miR-21 and miR-125 in 100 primary CRC specimens which were diagnosed and operated in West China Hospital between 2006 and 2007, in comparison with the corresponding normal mucosa specimens. The relationship between the expression of miRNAs and clinicopathological features was analyzed. Results The expression of miR-21 in CRC was up-regulated by 2.3 times compared to normal mucosa (P=0.025), while the expression of miR-125 was down-regulated by 3.3 times in comparison with normal mucosa(P=0.005). Furthermore, the expression of miR-21 was related to TNM stage (P=0.028) and local invasion (P=0.023). On the other hand, no significant relationship was found between the expression of miR-125 and clinicopathological features (P>0.05). Conclusion The over-expression of miR-21 may play a role in the development and progression of CRC, while miR-125 may not be related to the pathogenesis of CRC.