中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2008年
2期
128-129
,共2页
赵伟%杨国仁%于金明%宋现让%柳永蕾%赵书强%巩合义%王仁本
趙偉%楊國仁%于金明%宋現讓%柳永蕾%趙書彊%鞏閤義%王仁本
조위%양국인%우금명%송현양%류영뢰%조서강%공합의%왕인본
硝基咪唑类%同位素标记%氟放射性同位素%肿瘤%细胞低氧%小鼠,裸
硝基咪唑類%同位素標記%氟放射性同位素%腫瘤%細胞低氧%小鼠,裸
초기미서류%동위소표기%불방사성동위소%종류%세포저양%소서,라
Nitroimidazoles%Isotope labeling%Fluorine radioisotopes%Neoplasms%Cell hypoxia%Mice,nude
目的 探讨18F-氟赤式硝基咪唑(FETNIM)在肿瘤乏氧诊断中的应用价值.方法 30只SPCA-1人肺腺癌荷瘤BALB/c裸鼠采用随机数字表法分为A和B 2组(鼠数分别为16和14只).尾静脉注射37 MBq18F-FETNIM后A组分别于注射后0.5,1,2,3 h处死裸鼠,取血液、肺、心、肝、脾、肾、肿瘤等组织测量质量,用γ计数仪测定放射性计数,计算每克组织百分注射剂量率(%ID/g);B组用PO2微电极测量肿瘤组织内乏氧情况.结果 18F-FETNIM在肾中代谢最高,在脂肪和骨骼中代谢较低,肿瘤/正常组织的放射性比值较高,且随时间而增加,2 h达最高,肿瘤/血液放射性比值为1.69±0.37,肿瘤/肌肉放射性比值为1.57±0.47.HE染色显示瘤内有大量的乏氧坏死组织.组织内PO2微电极测量示肿瘤内均有乏氧,肿瘤内PO,1.1～27.7 mm Hg(1 mm Hg=0.133 kPa).结论 18F-FETNIM在荷瘤裸鼠体内具有较低的外周代谢,脂溶性较低,能被肿瘤乏氧组织摄取,可用于肿瘤乏氧诊断.
目的 探討18F-氟赤式硝基咪唑(FETNIM)在腫瘤乏氧診斷中的應用價值.方法 30隻SPCA-1人肺腺癌荷瘤BALB/c裸鼠採用隨機數字錶法分為A和B 2組(鼠數分彆為16和14隻).尾靜脈註射37 MBq18F-FETNIM後A組分彆于註射後0.5,1,2,3 h處死裸鼠,取血液、肺、心、肝、脾、腎、腫瘤等組織測量質量,用γ計數儀測定放射性計數,計算每剋組織百分註射劑量率(%ID/g);B組用PO2微電極測量腫瘤組織內乏氧情況.結果 18F-FETNIM在腎中代謝最高,在脂肪和骨骼中代謝較低,腫瘤/正常組織的放射性比值較高,且隨時間而增加,2 h達最高,腫瘤/血液放射性比值為1.69±0.37,腫瘤/肌肉放射性比值為1.57±0.47.HE染色顯示瘤內有大量的乏氧壞死組織.組織內PO2微電極測量示腫瘤內均有乏氧,腫瘤內PO,1.1～27.7 mm Hg(1 mm Hg=0.133 kPa).結論 18F-FETNIM在荷瘤裸鼠體內具有較低的外週代謝,脂溶性較低,能被腫瘤乏氧組織攝取,可用于腫瘤乏氧診斷.
목적 탐토18F-불적식초기미서(FETNIM)재종류핍양진단중적응용개치.방법 30지SPCA-1인폐선암하류BALB/c라서채용수궤수자표법분위A화B 2조(서수분별위16화14지).미정맥주사37 MBq18F-FETNIM후A조분별우주사후0.5,1,2,3 h처사라서,취혈액、폐、심、간、비、신、종류등조직측량질량,용γ계수의측정방사성계수,계산매극조직백분주사제량솔(%ID/g);B조용PO2미전겁측량종류조직내핍양정황.결과 18F-FETNIM재신중대사최고,재지방화골격중대사교저,종류/정상조직적방사성비치교고,차수시간이증가,2 h체최고,종류/혈액방사성비치위1.69±0.37,종류/기육방사성비치위1.57±0.47.HE염색현시류내유대량적핍양배사조직.조직내PO2미전겁측량시종류내균유핍양,종류내PO,1.1～27.7 mm Hg(1 mm Hg=0.133 kPa).결론 18F-FETNIM재하류라서체내구유교저적외주대사,지용성교저,능피종류핍양조직섭취,가용우종류핍양진단.
Objective The aim of this study was to investigate the value of18F-fluoroerythronitromidazole(FETNIM)in detecting tumor hypoxia in vivo.Methods BALB/c nude mice were xenografted with SPCA-1 lung cancer cells.Tumors were allowed to grow to a diameter about 10 mm.In group A (n=16),18F-FETNIM(37 MBq)was injected from tail vein.Biodistribution of 18F-FETNIM was studied at 0.5,1,2,and 3 h after tracer injection.Blood,muscle,heart,liver,kidney,fat,lung,and tumor were moved and weighted.The radioactivity was measured with percentage activity of injection dose per gram of tissue(%ID/g)by a γ-counting well.In group B(n=14),the intratumoral PO2 measurements were obrained by polar graphic oxygen needle probe.Results The radioactivity was highest in kidney and lowest in fat and bone matrix.The maximum tumor to blood(T/B)and tumor to muscle(T/M)ratios were 1.69±0. 37 and 1.57±0.47 at 2 h after injection.Intratumoral PO2 measurements were ranged from 1.1 to 27.7 mm Hg (1 mm Hg=0.133 kPa)and were 30.2 to 40.5 mm Hg for normal tissue.Conclusion A relatively high 18F-FETNIM retention ratio indicated18F-FETNIM to be a potential agent for detecting tumor hypoxia in vivo.
